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Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB5169 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Enolase 2/Neuron-specific Enolase Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Enolase 2/Neuron-specific Enolase in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P09104- Isotype
- IgG
- Antibody clone number
- 522601.211
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Enolase 2/Neuron-specific Enolase in SH-SY5Y Human Cell Line. Enolase 2/Neuron-specific Enolase was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human Enolase 2/Neuron-specific Enolase Monoclonal Antibody (Catalog # MAB5169) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
