ABIN108731

antibody from antibodies-online

Targeting: PSG1 CD66f, PBG1, PSBG1, PSGGA

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN108731

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN108731, RRID:AB_10953753

Product name

anti-Pregnancy Specific beta-1-Glycoprotein 5 (PSG5) antibody

Antibody type

Monoclonal

Antigen

immunisation with extracted protein of human PSG

Description

Protein G

Reactivity

Human

Isotype

IgG

Antibody clone number

BAP3

Vial size

100 uL

Concentration

1 mg/mL

Storage

short term: 2°C - 8°C, long term: -20°C

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow.

Thomas SN, Zhu F, Schnaar RL, Alves CS, Konstantopoulos K
The Journal of biological chemistry 2008 Jun 6;283(23):15647-55

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Murine CD9 is the receptor for pregnancy-specific glycoprotein 17.

Waterhouse R, Ha C, Dveksler GS
The Journal of experimental medicine 2002 Jan 21;195(2):277-82

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Pregnancy-specific glycoproteins function as immunomodulators by inducing secretion of IL-10, IL-6 and TGF-beta1 by human monocytes.

Snyder SK, Wessner DH, Wessells JL, Waterhouse RM, Wahl LM, Zimmermann W, Dveksler GS
American journal of reproductive immunology (New York, N.Y. : 1989) 2001 Apr;45(4):205-16

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Redefined nomenclature for members of the carcinoembryonic antigen family.

Beauchemin N, Draber P, Dveksler G, Gold P, Gray-Owen S, Grunert F, Hammarström S, Holmes KV, Karlsson A, Kuroki M, Lin SH, Lucka L, Najjar SM, Neumaier M, Obrink B, Shively JE, Skubitz KM, Stanners CP, Thomas P, Thompson JA, Virji M, von Kleist S, Wagener C, Watt S, Zimmermann W
Experimental cell research 1999 Nov 1;252(2):243-9

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The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues.

Hammarström S
Seminars in cancer biology 1999 Apr;9(2):67-81

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Highly specific monoclonal antibody demonstrates that pregnancy-specific glycoprotein (PSG) is limited to syncytiotrophoblast in human early and term placenta.

Zhou GQ, Baranov V, Zimmermann W, Grunert F, Erhard B, Mincheva-Nilsson L, Hammarström S, Thompson J
Placenta 1997 Sep;18(7):491-501

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[Isolation and purification of specific beta1-G-globulin].

Sokolov AV, Kozliaeva GA, Mesniankina NV, Tatarinov IuS
Voprosy meditsinskoi khimii 1978 Mar-Apr;24(2):240-4

Flow cytometry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

FACS analysis of CHO cells using BAP3. CHO cells were transiently transfected with an expression vector encoding a recombinant, transmembrane-anchored form of PSG1 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of BAP3 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with PSG1 transfected cells.

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1,3,5,7 or 8. Recognition of CEACAM3 and of a recombinant transmembrane-anchored PSG1 fusion protein was tested on stably transfected HeLa cells. Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEA-CAM1, 3 and 5: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3; PSG: BAP3; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified BAP3 was tested on all CEACAM transfectants. A positive signal was only obtained with PSG expressing cells (red curves).