Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- DM1211 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#DM1211, RRID:AB_1008777
- Product name
- anti CD66f / PSG1
- Antibody type
- Monoclonal
- Antigen
- Immunisation with extracted protein of human PSG
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- BAP3
- Vial size
- 0.1 mg
- Concentration
- 1,0 mg/ml
Submitted references Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow.
Thomas SN, Zhu F, Schnaar RL, Alves CS, Konstantopoulos K
The Journal of biological chemistry 2008 Jun 6;283(23):15647-55
The Journal of biological chemistry 2008 Jun 6;283(23):15647-55
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Fig.1: FACS analysis of CHO cells using BAP3 DM1211. CHO cells were transiently transfected with an expression vector encoding a recombinant, transmembrane-anchored form of PSG1(red curve) or an irrelevant protein (control transfectant: blackcurve). Binding of BAP3 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only withPSG1 transfected cells.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Fig2: Members of the CEA family were expressed on BOSC cells after transient transfection with expres-sion vectors containing either the cDNA of CEACAM1,3,5,7 or 8. Recognition of CEACAM3 and of arecombinant transmembrane-anchored PSG1 fusion protein was tested on stably transfected HeLa cells.Expression of the constructs was confirmed with monoclonal antibodies known to recognise the cor-responding proteins (CEACAM1, 3 and 5: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3; PSG: BAP3;green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For speci-ficity testing, protein G purified BAP3 was tested on all CEACAM transfectants. A positive signal was onlyobtained with PSG expressing cells (red curves).