Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-59453 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ANKEF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DCCKYIAQRG CDLKWKNLDH KTPRAVAKEG GFKAASKEIR RAERIANKLA RPGAKNPNPL WALRLHDWSV EREAFLREAF AVLDRGDGSI SKNDFVMVLE Highest antigen sequence identity to the following orthologs: Mouse - 80%, Rat - 81%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.6 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ANKEF1 in control (vector only transfected HEK293T lysate) and ANKEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a ANKEF1 Polyclonal Antibody (Product # PA5-59453).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ANKEF1 in human cell line A-431 shows positivity in nucleoli, plasma membrane & cytoplasm. Samples were probed using an ANKEF1 Polyclonal Antibody (Product # PA5-59453).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ANKEF1 in human testis tissue shows moderate cytoplasmic positivity in subset of cells in seminiferous ducts and in Leydig cells. Samples were probed using an ANKEF1 Polyclonal Antibody (Product # PA5-59453).