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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- PA5-59453 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ANKEF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: DCCKYIAQRG CDLKWKNLDH KTPRAVAKEG GFKAASKEIR RAERIANKLA RPGAKNPNPL WALRLHDWSV EREAFLREAF AVLDRGDGSI SKNDFVMVLE Highest antigen sequence identity to the following orthologs: Mouse - 80%, Rat - 81%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.6 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ANKEF1 in human cell line A-431 shows positivity in nucleoli, plasma membrane & cytoplasm. Samples were probed using an ANKEF1 Polyclonal Antibody (Product # PA5-59453).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of ANKEF1 in human cell line A-431 using ANKEF1 Polyclonal Antibody (Product # PA5-59453). Staining shows positivity in nucleoli, plasma membrane and cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ANKEF1 in human rectum using ANKEF1 Polyclonal Antibody (Product # PA5-59453) shows moderate cytoplasmic and nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ANKEF1 in human skeletal muscle using ANKEF1 Polyclonal Antibody (Product # PA5-59453) shows no positivity in myocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ANKEF1 in human fallopian tube using ANKEF1 Polyclonal Antibody (Product # PA5-59453) shows strong positivity in cilia in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ANKEF1 in human testis using ANKEF1 Polyclonal Antibody (Product # PA5-59453) shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts.
