- MA5-16987 - Invitrogen Antibodies SST antibody

Submitted references A point mutation in the Pdia6 gene results in loss of pancreatic β-cell identity causing overt diabetes.

Chhabra NF, Amend AL, Bastidas-Ponce A, Sabrautzki S, Tarquis-Medina M, Sachs S, Rubey M, Lorenz-Depiereux B, Feuchtinger A, Bakhti M, Lickert H, Przemeck GKH, Hrabě de Angelis M
Molecular metabolism 2021 Dec;54:101334

Pancreatic cancer triggers diabetes through TGF-β-mediated selective depletion of islet β-cells.

Parajuli P, Nguyen TL, Prunier C, Razzaque MS, Xu K, Atfi A
Life science alliance 2020 Jun;3(6)

Point mutations in the PDX1 transactivation domain impair human β-cell development and function.

Wang X, Sterr M, Ansarullah, Burtscher I, Böttcher A, Beckenbauer J, Siehler J, Meitinger T, Häring HU, Staiger H, Cernilogar FM, Schotta G, Irmler M, Beckers J, Wright CVE, Bakhti M, Lickert H
Molecular metabolism 2019 Jun;24:80-97

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: figs5 Suppl. Figure 5. Cells carrying PDX1 P33T/+ and PDX1 C18R/+ mutations are able to differentiate into beta-like cells. (A) Representative immunofluorescence staining for CPEP and SST in the XM001, PDX1 P33T/+ , and PDX1 C18R/+ cells at the S7 stage. Scale bar indicates 50 mum. (B) Insulin secretion assay for XM001, PDX1 P33T/+ , and PDX1 C18R/+ cells at the S7 stage (n = 3). figs5

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: figs9 Suppl. Figure 9. PDX1 is important for the generation of glucose-responsive beta-like cells . (A) Representative immunofluorescence staining for CPEP and SST in the XM001, PDX1 +/- , PDX1 P33T/P33T , and PDX1 C18R/C18R cells at the S7 stage. Scale bar indicates 50 mum. (B) C-peptide secretion assay for XM001, PDX1 +/- , PDX1 P33T/P33T , and PDX1 C18R/C18R cells at the S7 stage (n = 3). figs9

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Pdia6 point mutation results in a specific loss of beta-cells at postnatal stages. (A) Representative immunofluorescence images of insulin (green), glucagon (cyan), and somatostatin (red) in WT and Pdia6 F175S-/- mice at E18.5 and (B) quantification thereof. n = 3. Scale bar 30 mum. (C) Representative immunofluorescence images of insulin (green), glucagon (red), and somatostatin (cyan) in WT and Pdia6 F175S-/- mice at P14 and (D) quantification thereof. n = 3. (E) Representative immunofluorescence images of insulin (green) and glucagon (red) in WT and Pdia6 F175S-/- mice at P21. (F) Glucagon-to-insulin positive cell ratio at P21. n = 4-5. Scale bar = 50 mum. (G) UMAP plot from single cell RNA-Seq data [] on sorted islet cells in control and STZ-treated groups. (H) Violin plot displaying increased Pdia6 expression specifically in STZ-treated beta-cells. Error bars display +-SD. Differences were considered statistically significant at p < 0.05 using a two-tailed Student's t test (* p < 0.05, ** p < 0.01, *** p < 0.001). Figure 2

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2. Pancreatic ductal adenocarcinoma triggers selective depletion of beta-cells. (A, B) Formalin-fixed paraffin-embedded sections from KC or control mice (n = 6) were immunostained with anti-somatostatin (SST) or anti-pancreatic polypeptide (PP) antibodies and revealed by immunofluorescence. Representative pictures of normal, irregular, or remnant islets are shown (left). Percentage of irregular, near-PanIN, or remnant islets is shown. Somatostatin-positive (SST+) or pancreatic polypeptide-positive (PP+) cells in all islets from six different sections were counted, and results are presented as mean of the total number of SST+ or PP+ cells per islet (right) or as percentage of SST+ or PP+ cells relative to the total cell number in islets (right). Bar, 200 muM. (A, B) For the left graphs in (A, B), data are expressed as dot plot with a line at the median and whiskers showing SD. (A, B) For the right graphs in (A, B), data are expressed as mean +- SEM. Statistical significance was estimated by unpaired t test; ns, nonsignificant.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure S4. Specificity of pancreatic ductal adenocarcinoma-driven beta-cell depletion. (A, B, C, D) Formalin-fixed paraffin-embedded sections from control, pancreatitis (PPT), HFD-fed (HFD), or KC mice (n = 6) were immunostained with antibodies to insulin, glucagon, somatostatin, or PP and revealed by immunofluorescence. Representative pictures of normal or remnant islets are shown (left). INS+, GCG+, SST+, or PP+ cells in all islets from six different sections were counted, and results are presented as mean of the total number of hormone-positive cells per islet (right). Bar, 200 muM. Data are expressed as dot plot with a line at the median and whiskers showing SD. Statistical significance was estimated by two-way ANOVA. * P < 0.05; *** P < 0.001; ns, nonsignificant.

MA5-16987

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