MA5-17182

antibody from Invitrogen Antibodies

Targeting: SST SMST

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17182

Provider

Invitrogen Antibodies

Product name

Somatostatin Monoclonal Antibody (7G5)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-17182 targets SST in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human samples. The MA5-17182 immunogen is purified recombinant fragment of human SST (amino acids: 1-116) expressed in E. Coli. MA5-17182 detects SST which has a predicted molecular weight of approximately 12.7kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

7G5

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Human iPSC Modeling of Genetic Febrile Seizure Reveals Aberrant Molecular and Physiological Features Underlying an Impaired Neuronal Activity.

Scalise S, Zannino C, Lucchino V, Lo Conte M, Scaramuzzino L, Cifelli P, D'Andrea T, Martinello K, Fucile S, Palma E, Gambardella A, Ruffolo G, Cuda G, Parrotta EI
Biomedicines 2022 May 5;10(5)

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Transcriptomic and epigenomic dynamics associated with development of human iPSC-derived GABAergic interneurons.

Inglis GAS, Zhou Y, Patterson DG, Scharer CD, Han Y, Boss JM, Wen Z, Escayg A
Human molecular genetics 2020 Aug 29;29(15):2579-2595

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Flavonoid Glycosides of Polygonum capitatum Protect against Inflammation Associated with Helicobacter pylori Infection.

Zhang S, Mo F, Luo Z, Huang J, Sun C, Zhang R
PloS one 2015;10(5):e0126584

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SST using a SST monoclonal antibody (Product # MA5-17182) against a human SST recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SST using a SST monoclonal antibody (Product # MA5-17182) against a human SST recombinant protein.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Somatostatin was performed using 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Somatostatin Monoclonal Antibody (7G5) (Product # MA5-17182) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723), (1:3000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cytoplasmic localization. Panel e represents merged image for untreated U-2 OS cells showing no staining for Somatostatin. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HepG2 cells using SST monoclonal antibody (Product # MA5-17182) (green) and negative control (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 6 The staining of D cells of Gastric mucosa-associated lymphoid tissue (MALT) lymphoma (PVx200). Normal mice (without H . pylori infection) were assigned as a control group (CG group) and mice models infected with H . pylori were divided into model group (MG group, only treated with saline solution), triple combination therapy group (TG group, the daily medicine intake is 0.5 ug clarithromycin, 0.02 ug omeprazole, and 1 ug amoxicillin), low/middle/high concentrations of flavonoid glycoside group (LF/MF/HF group, treated with one daily dose of flavonoid glycoside at 32/64/128 ug), low/middle/high concentrations of flavonoid glycoside and common concentration of amoxicillin group (LFA/MFA/HFA group, treated with one daily dose of Flavonoid glycoside at 32/64/128 ug and amoxicillin at 1 ug). Analysis of G cells and somatostatin gray value in each group (n = 10). * P < 0.05 vs model group.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Types of interneurons generated from iPSCs. Immunofluorescence analysis of idNs stained with antibodies against interneuronal subtypes markers ( A ) somatostatin (SST), ( B ) calretinin (CALB2), and ( C ) calbindin (CALB1). In each group of images, WT cells are shown in the upper panel, while SCN1A M145T idNs are shown in the lower panel. White arrows in the merged images indicate neurons expressing the interneuronal makers indicated (63x magnification). ( D - F ) Quantification of percentage of MAP2 + neurons co-expressing interneuronal markers immunostained in panels ( A - C ). About 9-1% of idNs express SST and CALB2, while CALB1 is present in less than 1% percent of neurons. At least 200 cells were counted for each bar, and data are presented as mean +- SEM of two independent experiments.