13-9751-82

antibody from Invitrogen Antibodies

Targeting: SST SMST

Provider product page for 13-9751-82

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [3]
Comments [0]
Validations
Immunohistochemistry [1]
Other assay [2]

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Product number: 13-9751-82 - Provider product page
Provider: Invitrogen Antibodies
Product name: Somatostatin Monoclonal Antibody (ICDCLS), Biotin, eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: Description: The monoclonal antibody ICDCLS recognizes human, mouse, and rat Somatostatin. Somatostatin, also known as growth hormone-inhibiting hormone (GHIH) or somatotrophin release-inhibiting factor (SRIF), is a peptide hormone with two active forms (14 and 28 amino acids) produced by alternative cleavage of a single preproprotein. Somatostatin is secreted by the delta cells of the islet of Langerhans in the pancreas as well as by the hypothalamus and intestine. Somatostatin has a variety of functions, such as regulating the endocrine system and affecting neurotransmission and cell proliferation. Somatostatin also inhibits the release of numerous secondary hormones, such as insulin and glucagon. Somatostatin can be used to identify medium size aspiny neurons in the striatum, cortex, and hippocampus. Expression of somatostatin is present in hyperplasia of the pancreatic islets as well as islet cell tumors such as somatostatinomas. Applications Reported: This ICDCLS antibody has been reported for use in immunohistochemical staining, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This ICDCLS antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low pH antigen retrieval and can be used at less than or equal to 0.5 µg/mL. It is recommended that the antibody be carefully titrated for opitmal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity: Human, Mouse, Rat
Host: Mouse
Conjugate: Biotin
Isotype: IgG
Antibody clone number: ICDCLS
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: 4° C, store in dark, DO NOT FREEZE!

SST protein structure - 13-9751-82 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 DBP Alters alpha Cell and delta Cell Number and Size (A and B) Cell resolution reconstruction of pancreatic sections reveals no differences in alpha cell and beta cell mass in DBP +/+ and DBP -/- mice (A), quantified in bar graph (B) (scale bar represents 530 mum; representative images are shown; inset is a zoom showing maintenance of cellular resolution in a single image; n = 9-12 sections from 3 to 4 animals; unpaired t test). (C-I) Morphological analyses of DBP -/- islets (C) reveal increased alpha cell number (D) and decreased alpha cell size (E) (representative images shown in right panel), but normal area occupied (F). By contrast, beta cell number is increased (G), although beta cell size (H) (representative images shown in right panel) and area (I) are unchanged (scale bar in D represents 85 mum; scale bars in E and H represent 10 mum; n = 24-45 islets from 3 to 4 animals; E and H are zooms of C to better show alpha cell and beta cell size; DAPI is shown in blue; unpaired t test). (J-M) delta cell proportion (J and K; n = 55-79 islets from 4 to 5 animals) and size (L and M; n = 29 to 30 islets from 3 animals) are decreased in DBP -/- islets (scale bar in J represents 85 mum; scale bar in L represents 10 mum; representative images are shown; L is a zoom of J to better show delta cell size; unpaired t test). (N) Expression levels of the alpha cell differentiation markers Arx , Pax6 , Pou3f4 , and Irx2 are similar in DBP +/- and DBP -/- islets (n = 3-10 animals;
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 LUXendin645 reveals GLP1R expression in a subpopulation of alpha-cells. a - c LUXendin645 labeling is widespread throughout the intact islet, co-localizing predominantly with beta-cells a and delta-cells b , but less so with alpha-cells c stained for insulin (INS), somatostatin (SST), and glucagon (GCG), respectively ( n = 18 islets, seven animals, three separate islet preparations) (scale bar = 26 um). d Following dissociation of islets into cell clusters, LUXendin645 labeling can be more accurately quantified (arrows highlight cells selected for zoom-in) (scale bar = 26 um). e Zoom-in of d showing a LUXendin645 - (left) and LUXendin645 + (right) alpha-cell (arrows highlight non-labeled cell membrane, which is not bounded by a beta-cell) (scale bar = 26 um). f Box-and-whiskers plot showing proportion of beta-cells (INS) and alpha-cells (GCG) co-localized with LUXendin645 ( n = 18 cell clusters, ten animals, three separate islet preparations) (box and whiskers plot shows range and median; mean is shown by a plus symbol). g Ins1Cre Thor ;R26 mT/mG dual fluorophore reporter islets express tdTomato until Cre-mediated replacement with mGFP, allowing identification of beta-cells (~80% of the islet population) and non-beta-cells for live imaging (scale bar = 26 um). LUXendin645 (LUX645) highlights GLP1R expression in nearly all beta-cells but relatively few non-beta-cells ( n = 31 islets, six animals, three separate islet preparations). h A zoom-in of the islet in g showing
Conjugate: Biotin