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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- V7439 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MTAP Antibody
- Antibody type
- Monoclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Human, Other
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- MTAP/3137R
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)
- Storage
- Store the recombinant MTAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HeLa, A431, HepG2, HAP1 and U937 cell lysate with recombinant MTAP antibody. Expected molecular weight: 26-38 kDa (multiple isoforms).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HeLa, A431, HepG2, HAP1 and U937 cell lysate with recombinant MTAP antibody. Expected molecular weight: 26-38 kDa (multiple isoforms).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HT-29 and HEK293 cell lysate with recombinant MTAP antibody. Expected molecular weight: 26-38 kDa (multiple isoforms).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human kidney with recombinant MTAP antibody. Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human kidney with recombinant MTAP antibody. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human kidney with recombinant MTAP antibody. Required HIER: steam section in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free recombinant MTAP antibody (clone MTAP/3137R) as confirmation of integrity and purity.
