TA808440

antibody from OriGene

Targeting: NKX2-1 BCH, NKX2A, TITF1, TTF-1, TTF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA808440

Provider

OriGene

Proper citation

OriGene Cat#TA808440, RRID:AB_2628872

Product name

NKX2-1 mouse monoclonal antibody, clone OTI10F8 (formerly 10F8)

Antibody type

Monoclonal

Description

NKX2-1 mouse monoclonal antibody, clone OTI10F8 (formerly 10F8)

Host

Mouse

Conjugate

Unconjugated

Epitope

NKX2-1

Isotype

IgG

Antibody clone number

OTI10F8

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NKX2-1 (RC217520, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NKX2-

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunocytochemistry staining of NCI-H1975 cells using anti-NKX2-1 mouse monoclonal antibody (TA808440). The right is A549 cells as negative control.

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunocytochemistry staining of TT cells using anti-NKX2-1 mouse monoclonal antibody (TA808440). The right is A549 cells as negative control.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-NKX2-1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120?‚°C for 3 min, TA808440)(1:150)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NKX2-1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808440)(1:150)

Validation comment

IHC