Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- V3655 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NKX2.1 Antibody / TTF1
- Antibody type
- Monoclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- NX2.1/1855R
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)
- Storage
- Store the recombinant NKX2.1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC: Formalin-fixed, paraffin-embedded human thyroid stained with recombinant NKX2.1 antibody (clone NX2.1/1855R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC: Formalin-fixed, paraffin-embedded human lung adenocarcinoma stained with recombinant NKX2.1 antibody (clone NX2.1/1855R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free recombinant NKX2.1 antibody (clone NX2.1/1855R) as confirmation of integrity and purity.