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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab132947 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant TTF1 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human TTF1 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.Application:WB: 1/500 Ð 1/1000IHC: 1/50-1/100ICC/IF: 1/50-1/200Protein FunctionTranscription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 6-57- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,4 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant TTF1 Antibody (Ab132947) - Western Blot All lanes: Recombinant TTF1 Antibody (Ab132947) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 37 kDa Exposure time: 2.6 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant TTF1 Antibody (Ab132947) - IF Immunocytochemistry analysis of Thyroid Transcription Factor 1 (green) in HeLa cells using Recombinant TTF1 Antibody (Ab132947) and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant TTF1 Antibody (Ab132947) - IHC Immunohistochemistry analysis of paraffin-embedded Human thyroid using Recombinant TTF1 Antibody (Ab132947). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
