Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Other assay [2]
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Validation data
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- Product number
- STJ72909 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-Fgf14-Mouse antibody (N-Term) (STJ72909)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-Fgf14-Mouse (N-Term) is suitable for use in ELISA research applications.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
REQHWDRPSASR
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20 on receipt and minimise freeze-thaw cycles.
- Handling
- NA
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Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ72909 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ72909 (1:5000) immunostaining of FGF14-positive axon initial segments in cryosection of an immersion-fixed (1% PFA/0. 5% methanol) human hypothalamus*. Antigen retrieval with citrate buffer pH 6 at 80C for 30min. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method. Data obtained by Drs. Éva Rumpler and Erik Hrabovszky, Inst, Exp, Med, Budapest, Hungary.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA