HPA000834

G6PD antibody from Atlas Antibodies

G6PD1

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Enhanced validation

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Enhanced validation

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Antibody Data

Product number

HPA000834

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA000834, RRID:AB_611519

Product name

Anti-G6PD

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

FQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWL
FRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKA
TPEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHM
NALH


Isotype

IgG

Vial size

100 µl

Storage

Store at +4¡C for short term storage. Long time storage is recommended at -20¡C.

References

Global variability in gene expression and alternative splicing is modulated by mitochondrial content
Guantes R, Rastrojo A, Neves R, Lima A, Aguado B, Iborra F
Genome Research 2015 May;25(5):633-644

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TAp73 enhances the pentose phosphate pathway and supports cell proliferation
Du W, Jiang P, Mancuso A, Stonestrom A, Brewer M, Minn A, Mak T, Wu M, Yang X
Nature Cell Biology 2013 June;15(8):991-1000

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Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model
Kato B, Nicholson G, Neiman M, Rantalainen M, Holmes C, Barrett A, Uhlén M, Nilsson P, Spector T, Schwenk J
Proteome Science 2011 ;9(1):73

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line A-549.

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines A-549 and HEK293 using Anti-G6PD antibody. Corresponding G6PD RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows low expression as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human testis and kidney tissues using Anti-G6PD antibody. Corresponding G6PD RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more