MA5-15918

antibody from Invitrogen Antibodies

Targeting: G6PD G6PD1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15918

Provider

Invitrogen Antibodies

Product name

G6PD Monoclonal Antibody (5E12)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15918 targets G6PD in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human samples. The MA5-15918 immunogen is purified recombinant fragment of human G6PD expressed in E. Coli. . MA5-15918 detects G6PD which has a predicted molecular weight of approximately 59kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5E12

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Placental growth factor regulates the pentose phosphate pathway and antioxidant defense systems in human retinal endothelial cells.

Saddala MS, Lennikov A, Huang H
Journal of proteomics 2020 Apr 15;217:103682

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of G6PD was performed using A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with G6PD Monoclonal Antibody (5E12) (Product # MA5-15918) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing Nuclear and Cytoplasmic localization of G6PD. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of MCF-7 cells using G6PD monoclonal antibody (Product # MA5-15918) (green) and negative control (red).