MA1-16637

antibody from Invitrogen Antibodies

Targeting: MYC bHLHe39, c-Myc, MYCC

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-16637

Provider

Invitrogen Antibodies

Product name

c-Myc Monoclonal Antibody (9E11)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Suggested positive control: antigen standard for MYC (transient overexpression lysate), HL-60 cells or breast tumors, HL-60 whole cell lysate, human breast carcinoma whole cell lysate.

Reactivity

Human, Mouse, Rat, Chicken/Avian, Yeast

Host

Mouse

Isotype

IgG

Antibody clone number

9E11

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Isolation, culture, and characterization of chicken intestinal epithelial cells.

Ghiselli F, Rossi B, Felici M, Parigi M, Tosi G, Fiorentini L, Massi P, Piva A, Grilli E
BMC molecular and cell biology 2021 Feb 12;22(1):12

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Tupaia OASL1 Promotes Cellular Antiviral Immune Responses by Recruiting MDA5 to MAVS.

Yao YL, Yu D, Xu L, Gu T, Li Y, Zheng X, Bi R, Yao YG
Journal of immunology (Baltimore, Md. : 1950) 2020 Dec 15;205(12):3419-3428

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Loss of RIG-I leads to a functional replacement with MDA5 in the Chinese tree shrew.

Xu L, Yu D, Fan Y, Peng L, Wu Y, Yao YG
Proceedings of the National Academy of Sciences of the United States of America 2016 Sep 27;113(39):10950-5

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LRIG1 opposes epithelial-to-mesenchymal transition and inhibits invasion of basal-like breast cancer cells.

Yokdang N, Hatakeyama J, Wald JH, Simion C, Tellez JD, Chang DZ, Swamynathan MM, Chen M, Murphy WJ, Carraway Iii KL, Sweeney C
Oncogene 2016 Jun 2;35(22):2932-47

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Mcm10 self-association is mediated by an N-terminal coiled-coil domain.

Du W, Josephrajan A, Adhikary S, Bowles T, Bielinsky AK, Eichman BF
PloS one 2013;8(7):e70518

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The roles of the catalytic and noncatalytic activities of Rpd3L and Rpd3S in the regulation of gene transcription in yeast.

Yeheskely-Hayon D, Kotler A, Stark M, Hashimshony T, Sagee S, Kassir Y
PloS one 2013;8(12):e85088

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Trypanosoma brucei 14-3-3I and II proteins predominantly form a heterodimer structure that acts as a potent cell cycle regulator in vivo.

Inoue M, Yasuda K, Uemura H, Yasaka N, Schnaufer A, Yano M, Kido H, Kohda D, Doi H, Fukuma T, Tsuji A, Horikoshi N
Journal of biochemistry 2013 May;153(5):431-9

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MYC expression and distribution in normal mature lymphoid cells.

Cattoretti G
The Journal of pathology 2013 Feb;229(3):430-40

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Recruitment of Rpd3 to the telomere depends on the protein arginine methyltransferase Hmt1.

Milliman EJ, Yadav N, Chen YC, Muddukrishna B, Karunanithi S, Yu MC
PloS one 2012;7(8):e44656

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Widespread mRNA association with cytoskeletal motor proteins and identification and dynamics of myosin-associated mRNAs in S. cerevisiae.

Casolari JM, Thompson MA, Salzman J, Champion LM, Moerner WE, Brown PO
PloS one 2012;7(2):e31912

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Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components.

Kanke M, Kodama Y, Takahashi TS, Nakagawa T, Masukata H
The EMBO journal 2012 May 2;31(9):2182-94

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First-in-class small molecule inhibitors of the single-strand DNA cytosine deaminase APOBEC3G.

Li M, Shandilya SM, Carpenter MA, Rathore A, Brown WL, Perkins AL, Harki DA, Solberg J, Hook DJ, Pandey KK, Parniak MA, Johnson JR, Krogan NJ, Somasundaran M, Ali A, Schiffer CA, Harris RS
ACS chemical biology 2012 Mar 16;7(3):506-17

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Genomic insights of protein arginine methyltransferase Hmt1 binding reveals novel regulatory functions.

Milliman EJ, Hu Z, Yu MC
BMC genomics 2012 Dec 26;13:728

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CDK promotes interactions of Sld3 and Drc1 with Cut5 for initiation of DNA replication in fission yeast.

Fukuura M, Nagao K, Obuse C, Takahashi TS, Nakagawa T, Masukata H
Molecular biology of the cell 2011 Jul 15;22(14):2620-33

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Auxin-inducible protein depletion system in fission yeast.

Kanke M, Nishimura K, Kanemaki M, Kakimoto T, Takahashi TS, Nakagawa T, Masukata H
BMC cell biology 2011 Feb 11;12:8

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The interaction between AID and CIB1 is nonessential for antibody gene diversification by gene conversion or class switch recombination.

Demorest ZL, MacDuff DA, Brown WL, Morham SG, Parise LV, Harris RS
PloS one 2010 Jul 20;5(7):e11660

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Defects in DNA ligase I trigger PCNA ubiquitylation at Lys 107.

Das-Bradoo S, Nguyen HD, Wood JL, Ricke RM, Haworth JC, Bielinsky AK
Nature cell biology 2010 Jan;12(1):74-9; sup pp 1-20

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The Saccharomyces cerevisiae Esc2 and Smc5-6 proteins promote sister chromatid junction-mediated intra-S repair.

Sollier J, Driscoll R, Castellucci F, Foiani M, Jackson SP, Branzei D
Molecular biology of the cell 2009 Mar;20(6):1671-82

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Structural basis for DNA binding by replication initiator Mcm10.

Warren EM, Vaithiyalingam S, Haworth J, Greer B, Bielinsky AK, Chazin WJ, Eichman BF
Structure (London, England : 1993) 2008 Dec 10;16(12):1892-901

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A Sveinsson's chorioretinal atrophy-associated missense mutation in mouse Tead1 affects its interaction with the co-factors YAP and TAZ.

Kitagawa M
Biochemical and biophysical research communications 2007 Oct 5;361(4):1022-6

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Protein-tyrosine phosphatase PCP-2 inhibits beta-catenin signaling and increases E-cadherin-dependent cell adhesion.

Yan HX, Yang W, Zhang R, Chen L, Tang L, Zhai B, Liu SQ, Cao HF, Man XB, Wu HP, Wu MC, Wang HY
The Journal of biological chemistry 2006 Jun 2;281(22):15423-33

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A conserved Hsp10-like domain in Mcm10 is required to stabilize the catalytic subunit of DNA polymerase-alpha in budding yeast.

Ricke RM, Bielinsky AK
The Journal of biological chemistry 2006 Jul 7;281(27):18414-25

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Mcm10 regulates the stability and chromatin association of DNA polymerase-alpha.

Ricke RM, Bielinsky AK
Molecular cell 2004 Oct 22;16(2):173-85

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Activin isoforms signal through type I receptor serine/threonine kinase ALK7.

Tsuchida K, Nakatani M, Yamakawa N, Hashimoto O, Hasegawa Y, Sugino H
Molecular and cellular endocrinology 2004 May 31;220(1-2):59-65

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Genome-wide localization of the nuclear transport machinery couples transcriptional status and nuclear organization.

Casolari JM, Brown CR, Komili S, West J, Hieronymus H, Silver PA
Cell 2004 May 14;117(4):427-39

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Low expression levels of p27 correlate with loco-regional recurrence in nasopharyngeal carcinoma.

Hwang CF, Su CY, Huang SC, Huang CC, Fang FM, Lui CC, Chang HW, Cho CL
Cancer letters 2003 Jan 28;189(2):231-6

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Chromosomal gradient of histone acetylation established by Sas2p and Sir2p functions as a shield against gene silencing.

Kimura A, Umehara T, Horikoshi M
Nature genetics 2002 Nov;32(3):370-7

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of c-Myc in whole cell protein from PC3 (lane 1), U-2 OS (lane 2) and mouse testis (lane 3). Samples were incubated in c-Myc monoclonal antibody (Product # MA1-16637) using a dilution of 2.0 µg/mL followed by an anti-mouse HRP secondary antibody. Separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. Detection: chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-c-Myc Monoclonal Antibody (9E11) (Product # MA1-16637) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 60 µg (Lane 1), Myc-p65, 60 µg (Lane 2), Myc-p65, 30 µg (Lane 3), Myc-p65, 15 µg (Lane 4), Myc-p65, 7.5 µg (Lane 5),and 25 ng of Positope (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). A ~67 kDa band corresponding to Myc-p65-V5 observed in HEK293E transfected lysates on probing with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product #A28177, 1:20,000 dilution). Positope (Product # R900-50) is a 53 kDa recombinant protein consisting of multiple epitope tags, which has been used as a positive control for c-Myc tag detection (Lane 6).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of c-Myc in immersion fixed paraffin-embedded sections of human breast cancer. Samples were incubated in c-Myc monoclonal antibody (Product # MA1-16637) using a dilution of 1:600 overnight at 4 °C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent with DAB chromogen (brown) and counterstained with hematoxylin (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of c-Myc in U937 cells. Samples were incubated in c-Myc monoclonal antibody (Product # MA1-16637) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 647.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 11 MAPK1 expression by IF double staining 5d after seeding. CTR = cIECs cultured without growth factors - GF = cIECs cultured with growth factors a CTR contrast-phase image; b CTR FITC only; c CTR FITC+DAPI; d GF contrast-phase image; e GF FITC only; f) GF FITC+DAPI. Cells were double-stained for c-MYC and MAPK1