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- Antibody Data
- Antigen structure
- References [35]
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- Product number
- MA1-81357 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- c-Myc Monoclonal Antibody (9E10), HRP
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This product does not require protein digestion or antigen retrieval prior to staining of paraffin sections. Non-reducing conditions are recommended for Western blot applications. Mouse anti c-myc antibody, clone 9E10 detects the p62c-myc proto-oncogene protein, which is involved in the regulation of the cell cycle and cell growth.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Horseradish Peroxidase
- Isotype
- IgG
- Antibody clone number
- 9E10
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references The SUMOylation of TAB2 mediated by TRIM60 inhibits MAPK/NF-κB activation and the innate immune response.
Molecular Design, Optimization, and Genomic Integration of Chimeric B Cell Receptors in Murine B Cells.
AvrRpm1 Functions as an ADP-Ribosyl Transferase to Modify NOI Domain-Containing Proteins, Including Arabidopsis and Soybean RPM1-Interacting Protein4.
Identification of two novel Shank3 transcripts in the developing mouse neocortex.
Fulvestrant induces resistance by modulating GPER and CDK6 expression: implication of methyltransferases, deacetylases and the hSWI/SNF chromatin remodelling complex.
Activation of a PGC-1-related coactivator (PRC)-dependent inflammatory stress program linked to apoptosis and premature senescence.
Functional impact of Aurora A-mediated phosphorylation of HP1γ at serine 83 during cell cycle progression.
MAPK Hog1 closes the S. cerevisiae glycerol channel Fps1 by phosphorylating and displacing its positive regulators.
The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A.
Regulation of fertility, survival, and cuticle collagen function by the Caenorhabditis elegans eaf-1 and ell-1 genes.
Differential expression of MYC in H. pylori-related intestinal and diffuse gastric tumors.
Antibody library screens using detergent-solubilized mammalian cell lysates as antigen sources.
Selective interaction between Trf3 and Taf3 required for early development and hematopoiesis.
Relationship between EBV infection and expression of cellular proteins c-Myc, Bcl-2, and Bax in gastric carcinomas.
A Rictor-Myo1c complex participates in dynamic cortical actin events in 3T3-L1 adipocytes.
WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase.
DKC1 is a direct and conserved transcriptional target of c-MYC.
DDB1 functions as a linker to recruit receptor WD40 proteins to CUL4-ROC1 ubiquitin ligases.
Cytoplasmic localized ubiquitin ligase cullin 7 binds to p53 and promotes cell growth by antagonizing p53 function.
Negative regulation of TSC1-TSC2 by mammalian D-type cyclins.
Targeted ubiquitination of CDT1 by the DDB1-CUL4A-ROC1 ligase in response to DNA damage.
EHD2 and the novel EH domain binding protein EHBP1 couple endocytosis to the actin cytoskeleton.
Unconventional myosin Myo1c promotes membrane fusion in a regulated exocytic pathway.
N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.
14-3-3beta binds to and negatively regulates the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product, tuberin.
ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.
ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.
Cloning of mammalian Ire1 reveals diversity in the ER stress responses.
Tyrosine phosphorylation of selected secretory carrier membrane proteins, SCAMP1 and SCAMP3, and association with the EGF receptor.
Molecular cloning of caveolin-3, a novel member of the caveolin gene family expressed predominantly in muscle.
A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes.
A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes.
SNAP receptors implicated in vesicle targeting and fusion.
Epitope tagging and protein surveillance.
Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes.
Gu Z, Chen X, Yang W, Qi Y, Yu H, Wang X, Gong Y, Chen Q, Zhong B, Dai L, Qi S, Zhang Z, Zhang H, Hu H
Cellular & molecular immunology 2021 Aug;18(8):1981-1994
Cellular & molecular immunology 2021 Aug;18(8):1981-1994
Molecular Design, Optimization, and Genomic Integration of Chimeric B Cell Receptors in Murine B Cells.
Pesch T, Bonati L, Kelton W, Parola C, Ehling RA, Csepregi L, Kitamura D, Reddy ST
Frontiers in immunology 2019;10:2630
Frontiers in immunology 2019;10:2630
AvrRpm1 Functions as an ADP-Ribosyl Transferase to Modify NOI Domain-Containing Proteins, Including Arabidopsis and Soybean RPM1-Interacting Protein4.
Redditt TJ, Chung EH, Karimi HZ, Rodibaugh N, Zhang Y, Trinidad JC, Kim JH, Zhou Q, Shen M, Dangl JL, Mackey D, Innes RW
The Plant cell 2019 Nov;31(11):2664-2681
The Plant cell 2019 Nov;31(11):2664-2681
Identification of two novel Shank3 transcripts in the developing mouse neocortex.
Waga C, Asano H, Sanagi T, Suzuki E, Nakamura Y, Tsuchiya A, Itoh M, Goto Y, Kohsaka S, Uchino S
Journal of neurochemistry 2014 Jan;128(2):280-93
Journal of neurochemistry 2014 Jan;128(2):280-93
Fulvestrant induces resistance by modulating GPER and CDK6 expression: implication of methyltransferases, deacetylases and the hSWI/SNF chromatin remodelling complex.
Giessrigl B, Schmidt WM, Kalipciyan M, Jeitler M, Bilban M, Gollinger M, Krieger S, Jäger W, Mader RM, Krupitza G
British journal of cancer 2013 Nov 12;109(10):2751-62
British journal of cancer 2013 Nov 12;109(10):2751-62
Activation of a PGC-1-related coactivator (PRC)-dependent inflammatory stress program linked to apoptosis and premature senescence.
Gleyzer N, Scarpulla RC
The Journal of biological chemistry 2013 Mar 22;288(12):8004-8015
The Journal of biological chemistry 2013 Mar 22;288(12):8004-8015
Functional impact of Aurora A-mediated phosphorylation of HP1γ at serine 83 during cell cycle progression.
Grzenda A, Leonard P, Seo S, Mathison AJ, Urrutia G, Calvo E, Iovanna J, Urrutia R, Lomberk G
Epigenetics & chromatin 2013 Jul 5;6(1):21
Epigenetics & chromatin 2013 Jul 5;6(1):21
MAPK Hog1 closes the S. cerevisiae glycerol channel Fps1 by phosphorylating and displacing its positive regulators.
Lee J, Reiter W, Dohnal I, Gregori C, Beese-Sims S, Kuchler K, Ammerer G, Levin DE
Genes & development 2013 Dec 1;27(23):2590-601
Genes & development 2013 Dec 1;27(23):2590-601
The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A.
Moghe S, Jiang F, Miura Y, Cerny RL, Tsai MY, Furukawa M
Biology open 2012 Feb 15;1(2):82-91
Biology open 2012 Feb 15;1(2):82-91
Regulation of fertility, survival, and cuticle collagen function by the Caenorhabditis elegans eaf-1 and ell-1 genes.
Cai L, Phong BL, Fisher AL, Wang Z
The Journal of biological chemistry 2011 Oct 14;286(41):35915-35921
The Journal of biological chemistry 2011 Oct 14;286(41):35915-35921
Differential expression of MYC in H. pylori-related intestinal and diffuse gastric tumors.
Silva-Fernandes IJ, Alves MK, Lima VP, de Lima MA, Barros MA, Ferreira MV, Rabenhorst SH
Virchows Archiv : an international journal of pathology 2011 Jun;458(6):725-31
Virchows Archiv : an international journal of pathology 2011 Jun;458(6):725-31
Antibody library screens using detergent-solubilized mammalian cell lysates as antigen sources.
Cho YK, Shusta EV
Protein engineering, design & selection : PEDS 2010 Jul;23(7):567-77
Protein engineering, design & selection : PEDS 2010 Jul;23(7):567-77
Selective interaction between Trf3 and Taf3 required for early development and hematopoiesis.
Hart DO, Santra MK, Raha T, Green MR
Developmental dynamics : an official publication of the American Association of Anatomists 2009 Oct;238(10):2540-9
Developmental dynamics : an official publication of the American Association of Anatomists 2009 Oct;238(10):2540-9
Relationship between EBV infection and expression of cellular proteins c-Myc, Bcl-2, and Bax in gastric carcinomas.
Lima MA, Ferreira MV, Barros MA, Pardini MI, Ferrasi AC, Mota RM, Rabenhorst SH
Diagnostic molecular pathology : the American journal of surgical pathology, part B 2008 Jun;17(2):82-9
Diagnostic molecular pathology : the American journal of surgical pathology, part B 2008 Jun;17(2):82-9
A Rictor-Myo1c complex participates in dynamic cortical actin events in 3T3-L1 adipocytes.
Hagan GN, Lin Y, Magnuson MA, Avruch J, Czech MP
Molecular and cellular biology 2008 Jul;28(13):4215-26
Molecular and cellular biology 2008 Jul;28(13):4215-26
WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase.
Hu J, Zacharek S, He YJ, Lee H, Shumway S, Duronio RJ, Xiong Y
Genes & development 2008 Apr 1;22(7):866-71
Genes & development 2008 Apr 1;22(7):866-71
DKC1 is a direct and conserved transcriptional target of c-MYC.
Alawi F, Lee MN
Biochemical and biophysical research communications 2007 Nov 3;362(4):893-8
Biochemical and biophysical research communications 2007 Nov 3;362(4):893-8
DDB1 functions as a linker to recruit receptor WD40 proteins to CUL4-ROC1 ubiquitin ligases.
He YJ, McCall CM, Hu J, Zeng Y, Xiong Y
Genes & development 2006 Nov 1;20(21):2949-54
Genes & development 2006 Nov 1;20(21):2949-54
Cytoplasmic localized ubiquitin ligase cullin 7 binds to p53 and promotes cell growth by antagonizing p53 function.
Andrews P, He YJ, Xiong Y
Oncogene 2006 Aug 3;25(33):4534-48
Oncogene 2006 Aug 3;25(33):4534-48
Negative regulation of TSC1-TSC2 by mammalian D-type cyclins.
Zacharek SJ, Xiong Y, Shumway SD
Cancer research 2005 Dec 15;65(24):11354-60
Cancer research 2005 Dec 15;65(24):11354-60
Targeted ubiquitination of CDT1 by the DDB1-CUL4A-ROC1 ligase in response to DNA damage.
Hu J, McCall CM, Ohta T, Xiong Y
Nature cell biology 2004 Oct;6(10):1003-9
Nature cell biology 2004 Oct;6(10):1003-9
EHD2 and the novel EH domain binding protein EHBP1 couple endocytosis to the actin cytoskeleton.
Guilherme A, Soriano NA, Bose S, Holik J, Bose A, Pomerleau DP, Furcinitti P, Leszyk J, Corvera S, Czech MP
The Journal of biological chemistry 2004 Mar 12;279(11):10593-605
The Journal of biological chemistry 2004 Mar 12;279(11):10593-605
Unconventional myosin Myo1c promotes membrane fusion in a regulated exocytic pathway.
Bose A, Robida S, Furcinitti PS, Chawla A, Fogarty K, Corvera S, Czech MP
Molecular and cellular biology 2004 Jun;24(12):5447-58
Molecular and cellular biology 2004 Jun;24(12):5447-58
N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.
Wahl JK 3rd, Kim YJ, Cullen JM, Johnson KR, Wheelock MJ
The Journal of biological chemistry 2003 May 9;278(19):17269-76
The Journal of biological chemistry 2003 May 9;278(19):17269-76
14-3-3beta binds to and negatively regulates the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product, tuberin.
Shumway SD, Li Y, Xiong Y
The Journal of biological chemistry 2003 Jan 24;278(4):2089-92
The Journal of biological chemistry 2003 Jan 24;278(4):2089-92
ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.
Ohta T, Michel JJ, Schottelius AJ, Xiong Y
Molecular cell 1999 Apr;3(4):535-41
Molecular cell 1999 Apr;3(4):535-41
ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.
Ohta T, Michel JJ, Schottelius AJ, Xiong Y
Molecular cell 1999 Apr;3(4):535-41
Molecular cell 1999 Apr;3(4):535-41
Cloning of mammalian Ire1 reveals diversity in the ER stress responses.
Wang XZ, Harding HP, Zhang Y, Jolicoeur EM, Kuroda M, Ron D
The EMBO journal 1998 Oct 1;17(19):5708-17
The EMBO journal 1998 Oct 1;17(19):5708-17
Tyrosine phosphorylation of selected secretory carrier membrane proteins, SCAMP1 and SCAMP3, and association with the EGF receptor.
Wu TT, Castle JD
Molecular biology of the cell 1998 Jul;9(7):1661-74
Molecular biology of the cell 1998 Jul;9(7):1661-74
Molecular cloning of caveolin-3, a novel member of the caveolin gene family expressed predominantly in muscle.
Tang Z, Scherer PE, Okamoto T, Song K, Chu C, Kohtz DS, Nishimoto I, Lodish HF, Lisanti MP
The Journal of biological chemistry 1996 Jan 26;271(4):2255-61
The Journal of biological chemistry 1996 Jan 26;271(4):2255-61
A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes.
Gorsch LC, Dockendorff TC, Cole CN
The Journal of cell biology 1995 May;129(4):939-55
The Journal of cell biology 1995 May;129(4):939-55
A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes.
Gorsch LC, Dockendorff TC, Cole CN
The Journal of cell biology 1995 May;129(4):939-55
The Journal of cell biology 1995 May;129(4):939-55
SNAP receptors implicated in vesicle targeting and fusion.
Söllner T, Whiteheart SW, Brunner M, Erdjument-Bromage H, Geromanos S, Tempst P, Rothman JE
Nature 1993 Mar 25;362(6418):318-24
Nature 1993 Mar 25;362(6418):318-24
Epitope tagging and protein surveillance.
Kolodziej PA, Young RA
Methods in enzymology 1991;194:508-19
Methods in enzymology 1991;194:508-19
Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes.
Kari B, Lussenhop N, Goertz R, Wabuke-Bunoti M, Radeke R, Gehrz R
Journal of virology 1986 Nov;60(2):345-52
Journal of virology 1986 Nov;60(2):345-52
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Horseradish Peroxidase
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Horseradish Peroxidase
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Horseradish Peroxidase
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Genomic integration of chimeric B cell receptors (CBCR) by CRISPR-Cas9-mediated HDR. (A) Schematic shows the design of the CBCR, which consists of an scFv-based antigen recognition domain (specific for a hen-egg lysozyme [HEL]) (orange), an extracellular spacer with a detection tag, a transmembrane (TM) domain (light blue), and endodomains (blue, purple). scFV, single chain variable fragment; VL, light chain variable domain; VH, heavy chain variable domain. (B) Shown is the gRNA sequence (blue) for Cas9-targeting of the safe-harbor locus Rosa26 locus; also shown is the corresponding PAM sequence (orange) and the beginning of the homology arms in the HDR template (light gray). The two blue arrows indicate the predicted Cas9 double-stranded break site. (C) CRISPR-Cas9-mediated HDR for genomic integration of CBCR construct into the Rosa26 locus. The PCR-linearized donor template contains a GFP reporter gene followed by a T2A coding sequence, the Vkappa signal peptide, the CBCR cassette and a poly-A sequence all under the control of a CMV promoter and flanked by sequences homologous to the Rosa26 locus next to the gRNA target site of 1.4 and 1.5 kb, respectively (light purple). T2A: the self-cleaving thosea asigna virus 2A sequence, pA: SV40 polyA sequence. (D) Schematic shows the format of the CBCR antigen binding domain including a linker with a detection tag, either Myc or Flag epitope. (E) Cells were enriched for GFP (488 nm) expression via FACS. Graph shows ELISA re
- Conjugate
- Horseradish Peroxidase
