Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- IC3696N - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal c-Myc Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects endogenous human c-Myc and c-Myc tagged proteins in Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Near infrared dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references TGF-β inhibits IL-7-induced proliferation in memory but not naive human CD4+ T cells.
Nguyen TP, Sieg SF
Journal of leukocyte biology 2017 Aug;102(2):499-506
Journal of leukocyte biology 2017 Aug;102(2):499-506
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of c-Myc in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human c-Myc Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # IC3696N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.