AHO0062

antibody from Invitrogen Antibodies

Targeting: MYC bHLHe39, c-Myc, MYCC

Provider product page for AHO0062

Western blot

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Immunoelectron microscopy

Other assay

Antibody data

Antibody Data
Antigen structure
References [45]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]
Other assay [4]

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Product number: AHO0062 - Provider product page
Provider: Invitrogen Antibodies
Product name: c-Myc Monoclonal Antibody (9E10.3)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: This antibody detects an epitope at aa 410-419. Staining of formalin-fixed paraffin embedded tissue sections requires boiling the sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes. Recommended positive controls include Raji cells and human breast carcinoma.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 9E10.3
Vial size: 500 µL
Concentration: 0.2 mg/mL
Storage: 4° C

MYC protein structure - AHO0062 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 ChIP mapping of genomic Zap1 binding sites. Zap1 myc-tagged strain CJN1688 versus untagged wild-type strain DAY185 immunoprecipitation binding data were performed under biofilm conditions. The x -axis represents ORF chromosomal locations (See Dataset S6 , sheet 1 for exact location values). The y -axis is the Agilent normalized enrichment value (log2) for binding of Zap1 (See Dataset S6 , sheet 1 for exact enrichment values). Zap1-myc strain (blue line) and untagged wild-type (red line) ChIP-chip array binding data were mapped and plotted onto the chromosomes containing ZRT1 and PRA1 located on Chromosome 4 (A), ZRT2 located on Chromosome 2 (B), ZRT3 located on Chromosome 2 (C), CSH1 located on Chromosome 1 (D), IFD6 located on Chromosome 1 (E), and itself ZAP1 located on Chromosome 4 (F) using ChipView v0.954. The promoters of these genes show significant peak enrichment (determined using Agilent Chip Analytics software v1.2) for the binding of Zap1. The blue track under the peak indicates that the Agilent segment p -value (-log10) for the binding of Zap1 is significant (See Dataset S6 , sheet 1 for actual segment p -values). Genes plotted above the bold line read in the sense direction; genes plotted below the bold line read in the antisense direction. Identical binding sites with similar peak enrichment values were observed for the independently isolated Zap1 myc-tagged strain CJN1694 versus untagged wild-type strain DAY185 (unpublished data).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 ChIP mapping of genomic Zap1 binding sites. Zap1 myc-tagged strain CJN1688 versus untagged wild-type strain DAY185 immunoprecipitation binding data were performed under biofilm conditions. The x -axis represents ORF chromosomal locations (See Dataset S6 , sheet 1 for exact location values). The y -axis is the Agilent normalized enrichment value (log2) for binding of Zap1 (See Dataset S6 , sheet 1 for exact enrichment values). Zap1-myc strain (blue line) and untagged wild-type (red line) ChIP-chip array binding data were mapped and plotted onto the chromosomes containing ZRT1 and PRA1 located on Chromosome 4 (A), ZRT2 located on Chromosome 2 (B), ZRT3 located on Chromosome 2 (C), CSH1 located on Chromosome 1 (D), IFD6 located on Chromosome 1 (E), and itself ZAP1 located on Chromosome 4 (F) using ChipView v0.954. The promoters of these genes show significant peak enrichment (determined using Agilent Chip Analytics software v1.2) for the binding of Zap1. The blue track under the peak indicates that the Agilent segment p -value (-log10) for the binding of Zap1 is significant (See Dataset S6 , sheet 1 for actual segment p -values). Genes plotted above the bold line read in the sense direction; genes plotted below the bold line read in the antisense direction. Identical binding sites with similar peak enrichment values were observed for the independently isolated Zap1 myc-tagged strain CJN1694 versus untagged wild-type strain DAY185 (unpublished data).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6. Split YFP-based bimolecular fluorescence complementation and co-IP analyses. (A and B) Laser scanning microscopy images were taken from mesophyll cells of N. benthamiana transiently co-expressing YFP N- or C-half fusion proteins that are labeled as -YFP N and -YFP C respectively. Fluorescence images for YFP, chlorophyll, and the merged signals are shown. Scale bars represent 5 um. (C) Co-IP of the Arabidopsis SecY1 complex from tobacco leaves after co-infiltration and transient expression of YFP N -tagged SecY1 and GFP fusion proteins that were led by PsbO1 1-85 (lane 2) or full-length PsbO1 (PsbO1 1-332 ) (lane 3). Lane 1 is the wild-type control. YFP N was also Myc tagged. Anti-HSP90C antibody recognizes both Arabidopsis and tobacco chloroplast HSP90C. It should be noted that all full-length GFP fusion constructs are based on the pEGAD vector and there is a 27 amino acid linker region after the GFP C-terminus yielding a slightly larger GFP fusion protein than normally expected. Construct details are also shown in Fig. 1A . (D) Co-IP of the SecY1 complex from 12-day-old SECY1-T7/ secy1 Arabidopsis seedlings using anti-T7 antibody agarose. Wild-type (WT) seedlings were used as a negative control. Anti-HSP90C and anti-T7 antibodies were used to detect HSP90C and SecY1 proteins in both total input and the pull-down (IP) samples.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 3. The role of Tye7 in lineage-specific selection on glycolysis. ( A ) Cells were serially diluted fivefold onto solid minimal media containing 2% glucose as a carbon source with and without antimycin A to inhibit respiration and force cells to rely on glycolysis. Plates were grown at 30 degC for 72 h and pictures were taken with identical aperture and exposure settings. The results show that Tye7 is needed in C. dubliniensis for growth on antimycin A. ( B ) Schematic of immunoprecipitation experiments performed in the interspecies hybrid where one allele of the C. dubliniensis Tye7 was epitope-tagged. The ChIP-seq reads from the tagged hybrid were aligned to the concatenated genome of the two species and peaks were called in intergenic regions using MACS2 (). Instances of allelic bias were detected using the same methods used for identifying ASE ( Materials and Methods ). ( C and D ) ChIP-seq of Tye7 in the interspecies hybrid shows differential binding to promoters of glycolysis genes PFK1 and HXK2 , respectively. The boxes depict the 1,000 base pair regions of the intergenic region upstream of the glycolysis genes in C. albicans ( Left ) and C. dubliniensis ( Right ), centered around the called ChIP peak. Darker- and lighter-colored lines represent ChIP-seq reads obtained from two replicate experiments in the interspecies hybrid. Magenta lines depict reads that map to the C. albicans genome, whereas green lines depict reads that map to the C. dubliniensis genome. Gray