MA1-21316-BTIN

antibody from Invitrogen Antibodies

Targeting: MYC bHLHe39, c-Myc, MYCC

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA1-21316-BTIN

Provider

Invitrogen Antibodies

Product name

Anti-Myc Tag Monoclonal Antibody (Myc.A7), Biotin

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA1-21316-BTIN has been successfully used for Western blot, ELISA, ICC/IF and IP applications.

Host

Mouse

Conjugate

Biotin

Isotype

IgG

Antibody clone number

Myc.A7

Vial size

50 µL

Concentration

1 mg/mL

Storage

4° C, do not freeze

References

Submitted references

DNA Replication Stress Phosphoproteome Profiles Reveal Novel Functional Phosphorylation Sites on Xrs2 in Saccharomyces cerevisiae.

Huang D, Piening BD, Kennedy JJ, Lin C, Jones-Weinert CW, Yan P, Paulovich AG
Genetics 2016 May;203(1):353-68

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a biotinylated Myc Epitope Tag monoclonal antibody (Product # MA1-21316-BTIN) at a dilution of 1:1000 for 1 hour at room temperature, followed by Streptavidin-HRP (Product # 21126) at a dilution of 1:20,000 for 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078).

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1:1000 (1 µg/mL) Ab dilution probed against HEK293 cells transfected with Myc-tagged protein vector; untransfected (1) and transfected (2).

Conjugate

Biotin