MA1-21316-D800

antibody from Invitrogen Antibodies

Targeting: MYC bHLHe39, c-Myc, MYCC

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-21316-D800

Provider

Invitrogen Antibodies

Product name

Anti-Myc Tag Monoclonal Antibody (Myc.A7), DyLight 800 4X PEG

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA1-21316-D800 detects Myc tag from various samples. MA1-21316-D800 has been successfully used for fluorescent detection of Myc tagged proteins in Western Blotting. DyLight 800 4X PEG has an excitation/emission of 783/797 nm.

Host

Mouse

Isotype

IgG

Antibody clone number

Myc.A7

Vial size

50 µL

Concentration

1 mg/mL

Storage

4° C, do not freeze

References

Submitted references

DNA Replication Stress Phosphoproteome Profiles Reveal Novel Functional Phosphorylation Sites on Xrs2 in Saccharomyces cerevisiae.

Huang D, Piening BD, Kennedy JJ, Lin C, Jones-Weinert CW, Yan P, Paulovich AG
Genetics 2016 May;203(1):353-68

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Myc Epitope Tag was performed by loading 2 µg of E. coli lysate containing a Myc-epitope tagged protein onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a low fluorescence PVDF membrane (Product # 22860) and blocked with SEA BLOCK (Product # 37527) for at least 1 hour. The membrane was probed with a DyLight 800-conjugated Myc Epitope Tag monoclonal antibody (Product # MA1-21316-D800) at a dilution of 1:500 overnight at 4°C on a rocking platform and washed in TBS-0.1% Tween-20. Fluorescent detection was performed using a near IR imaging system.