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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- V2745 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Myc Antibody
- Antibody type
- Monoclonal
- Antigen
- Amino acids AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA from the C-terminus was used as the immunogen for the Myc antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- SPM237
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Myc antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HeLa cell lysate (nuclear fraction) with Myc antibody (clone SPM237). Theoretical molecular weight: ~50kDa but routinely observed at 50~70kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human cervical carcinoma stained with Myc antibody (SPM237).
