Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AP12777PU-N - Provider product page
- Provider
- OriGene
- Product name
- c-Myc (MYC) pThr58 rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- c-Myc (MYC) pThr58 rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MYC
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- (LEFT) Dot blot analysis of Phospho-MYC-Thr58 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. (RIGHT) Western blot analysis of Phospho-MYC-Thr58 Antibody in human TPA activated Hela cell line lysates. Phospho-MYC (arrow) was detected using the purified PAb. (0: without TPA; 1: 60ug/ml TPA, 15min; 2: 60ug/ml TPA, 30min; 3: 60ug/ml TPA, 45min; 4: 125ug/ml TPA, 15min; 5: 125ug/ml TPA, 30min; 6: 125ug/ml TPA, 45min; 7: 250ug/ml TPA, 15min; 8: 250ug/ml TPA, 30min; 9: 250ug/ml, 45min)
- Validation comment
- WB