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- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- R1182 - Provider product page
- Provider
- OriGene
- Product name
- c-Myc (MYC) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- c-Myc (MYC) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MYC
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Figure 1. Anti-Myc epitope tag polyclonal antibody detects both AMINO and CARBOXY terminal linked Myc-tagged recombinant proteins by Western blot. Polyclonal rabbit-anti-Myc epitope tag antibody was diluted to 1.0 ug/ml to detect either recombinant protein. 4-20% gradient gels were used to separate the proteins by SDS-PAGE. The proteins were transferred to nitrocellulose using standard methods. After blocking the membranes were probed with the primary antibody overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye? 800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (Green 800 nm channel). Pre-stained molecular weight markers are also shown (lane M, Red 700 nm channel). LICOR's Odyssey? Infrared Imaging System was used to scan and process the image.
- Validation comment
- WB
