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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA3-981 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Myc Tag Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA3-981 detects c-Myc tagged proteins. PA3-981 has been successfully used in immunofluorescence, immunoprecipitation, and Western blot procedures. By Western blot it detects a ~41 kDa protein representing c-Myc. The PA3-981 immunogen is a synthetic peptide corresponding to residues M E Q K L I S E E D L G C of human c-Myc.
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Lot Dependent
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Functional and molecular evidence of MaxiK channel beta1 subunit decrease with coronary artery ageing in the rat.
Nishimaru K, Eghbali M, Lu R, Marijic J, Stefani E, Toro L
The Journal of physiology 2004 Sep 15;559(Pt 3):849-62
The Journal of physiology 2004 Sep 15;559(Pt 3):849-62
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of c-Myc was performed by loading 25 µg of 293t cells transfected with c-Myc -MYCBP (lane 1), transfected with c-Myc -FLI1 (lane 2) and transfected with 12Tag (lane 3) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a c-Myc Epitope Tag polyclonal antibody (Product # PA3-981) at a dilution of 1:5000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). The image shows the target bands on c-Myc -MYCBP (19kDa), c-Myc -FLI1 (54kDa) and 12Tag (45kDa), which are consistent with predicted molecular weights for each target.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of cMyc-tagged GTPase in HeLa cells using Product # PA3-981 in green.
