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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- R953-25 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Myc Tag Monoclonal Antibody, FITC
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- NOTE: R953-25 was previously sold under product #46-0307. When re-ordering, please use #R953-25. R953-25 recognizes amino acid sequence: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-. This antibody has been tested in IF experiments using cultured CHO (Chinese Hamster ovary) cells expressing recombinant epitope-tagged fusion proteins. For IF, dilute the FITC-conjugated antibody in PBS containing 10% fetal bovine serum (FBS). The amount of antibody provided is sufficient for 25 immunostaining reactions using a 1 mL working solution per reaction. This antibody has also been tested against purified Positope™ control protein. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5.
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 50 µl
- Concentration
- 1 mg/mL
- Storage
- 4° C, store in dark
Submitted references Cyclin B3 controls anaphase onset independent of spindle assembly checkpoint in meiotic oocytes.
Casein kinase 1 (α, δ and ε) localize at the spindle poles, but may not be essential for mammalian oocyte meiotic progression.
Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion.
Zhang T, Qi ST, Huang L, Ma XS, Ouyang YC, Hou Y, Shen W, Schatten H, Sun QY
Cell cycle (Georgetown, Tex.) 2015;14(16):2648-54
Cell cycle (Georgetown, Tex.) 2015;14(16):2648-54
Casein kinase 1 (α, δ and ε) localize at the spindle poles, but may not be essential for mammalian oocyte meiotic progression.
Qi ST, Wang ZB, Huang L, Liang LF, Xian YX, Ouyang YC, Hou Y, Sun QY, Wang WH
Cell cycle (Georgetown, Tex.) 2015;14(11):1675-85
Cell cycle (Georgetown, Tex.) 2015;14(11):1675-85
Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion.
Asano S, Nemoto T, Kitayama T, Harada K, Zhang J, Harada K, Tanida I, Hirata M, Kanematsu T
Biology open 2014 May 8;3(6):463-74
Biology open 2014 May 8;3(6):463-74
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of c-Myc Epitope Tag, FITC conjugate was done on SH-SY5Y cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with c-Myc Epitope Tag, FITC conjugate Mouse Monoclonal Antibody (R95325, red histogram) or with mouse isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA and incubated at room temperature for 2 hours. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
