NBP2-52636-0.2mg

antibody from Novus Biologicals

Targeting: MYC bHLHe39, c-Myc, MYCC

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

NBP2-52636-0.2mg

Provider

Novus Biologicals

Product name

Rabbit Monoclonal c-Myc Antibody

Antibody type

Monoclonal

Description

Protein A purified. Epitope sequence, EQKLISEEDL, located in aa 410-419 of human c-myc protein.

Reactivity

Human, Yeast

Host

Rabbit

Isotype

IgG

Vial size

0.2 mg

Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: c-Myc Antibody (9E10) [NBP2-52636] - Analysis of HEK293 lysate spiked (A) and unspiked (B) with 5 ng multitag protein (35 ug protein in RIPA buffer). Lane C contains 25 ng purified multitag protein as positive control. Right Panel: (1 ug/ml) staining of Multitag protein at 25 ng (A), 10 ng (B) and 5 ng (C) amounts. For both, primary incubation was 1 hour. Detected by chemiluminescence.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: c-Myc Antibody (9E10) [NBP2-52636] - Supporting data for Anti-c-myc epitope tag [9E10]. ELISA of anti-epitope tag antibodies on multi-tag protein. Plate coated with 5ug/ml of Multitag protein. Primary antibody loaded at 1000ng/ml in first well and an 11 point serial dilution performed in duplicate for each antibody with last well left blank. Signal detection was performed using HRP-conjugated goat anti-mouse.