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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PAB0541 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0541, RRID:AB_982468
- Product name
- MYC (phospho T58) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of MYC.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A comparison of phosphospecific affinity reagents reveals the utility of recombinant Forkhead-associated domains in recognizing phosphothreonine-containing peptides.
Prolactin induces c-Myc expression and cell survival through activation of Src/Akt pathway in lymphoid cells.
Level of MYC overexpression in pediatric Burkitt's lymphoma is strongly dependent on genomic breakpoint location within the MYC locus.
p19ARF directly and differentially controls the functions of c-Myc independently of p53.
Venegas LA, Pershad K, Bankole O, Shah N, Kay BK
New biotechnology 2016 Sep 25;33(5 Pt A):537-43
New biotechnology 2016 Sep 25;33(5 Pt A):537-43
Prolactin induces c-Myc expression and cell survival through activation of Src/Akt pathway in lymphoid cells.
Domínguez-Cáceres MA, García-Martínez JM, Calcabrini A, González L, Porque PG, León J, Martín-Pérez J
Oncogene 2004 Sep 23;23(44):7378-90
Oncogene 2004 Sep 23;23(44):7378-90
Level of MYC overexpression in pediatric Burkitt's lymphoma is strongly dependent on genomic breakpoint location within the MYC locus.
Wilda M, Busch K, Klose I, Keller T, Woessmann W, Kreuder J, Harbott J, Borkhardt A
Genes, chromosomes & cancer 2004 Oct;41(2):178-82
Genes, chromosomes & cancer 2004 Oct;41(2):178-82
p19ARF directly and differentially controls the functions of c-Myc independently of p53.
Qi Y, Gregory MA, Li Z, Brousal JP, West K, Hann SR
Nature 2004 Oct 7;431(7009):712-7
Nature 2004 Oct 7;431(7009):712-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MYC (phospho T58) polyclonal antibody (Cat # PAB0541) in human TPA activated HeLa cell line lysates. Phospho-MYC (arrow) was detected using the purified PAb.(0: without TPA; 1: 60 ug/ml TPA, 15 min; 2: 60 ug/ml TPA, 30 min; 3: 60 ug/ml TPA, 45 min; 4: 125 ug/ml TPA, 15 min; 5: 125 ug/ml TPA, 30 min; 6: 125 ug/ml TPA, 45 min; 7: 250 ug/ml TPA, 15 min; 8: 250 ug/ml TPA, 30 min; 9: 250 ug/ml, 45 min).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of MYC (phospho T58) polyclonal antibody (Cat # PAB0541) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)
