Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB6763 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB6763, RRID:AB_10678479
- Product name
- MYC (phospho S62) monoclonal antibody, clone 33A12E10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against synthetic phosphopeptide of MYC.
- Isotype
- IgG
- Antibody clone number
- 33A12E10
- Storage
- Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Submitted references MiR-138 downregulates miRNA processing in HeLa cells by targeting RMND5A and decreasing Exportin-5 stability.
MYC-dependent regulation and prognostic role of CIP2A in gastric cancer.
CIP2A inhibits PP2A in human malignancies.
Multiple Ras-dependent phosphorylation pathways regulate Myc protein stability.
Li J, Chen Y, Qin X, Wen J, Ding H, Xia W, Li S, Su X, Wang W, Li H, Zhao Q, Fang T, Qu L, Shao N
Nucleic acids research 2014 Jan;42(1):458-74
Nucleic acids research 2014 Jan;42(1):458-74
MYC-dependent regulation and prognostic role of CIP2A in gastric cancer.
Khanna A, Böckelman C, Hemmes A, Junttila MR, Wiksten JP, Lundin M, Junnila S, Murphy DJ, Evan GI, Haglund C, Westermarck J, Ristimäki A
Journal of the National Cancer Institute 2009 Jun 3;101(11):793-805
Journal of the National Cancer Institute 2009 Jun 3;101(11):793-805
CIP2A inhibits PP2A in human malignancies.
Junttila MR, Puustinen P, Niemelä M, Ahola R, Arnold H, Böttzauw T, Ala-aho R, Nielsen C, Ivaska J, Taya Y, Lu SL, Lin S, Chan EK, Wang XJ, Grènman R, Kast J, Kallunki T, Sears R, Kähäri VM, Westermarck J
Cell 2007 Jul 13;130(1):51-62
Cell 2007 Jul 13;130(1):51-62
Multiple Ras-dependent phosphorylation pathways regulate Myc protein stability.
Sears R, Nuckolls F, Haura E, Taya Y, Tamai K, Nevins JR
Genes & development 2000 Oct 1;14(19):2501-14
Genes & development 2000 Oct 1;14(19):2501-14
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot from crude cell extracts of AGS (gastric adenocarcinoma) cells with MYC (phospho S62) monoclonal antibody, clone 33A12E10 (Cat # MAB6763).Lane 1 : Scrambled siRNA was introduced into the cells as a negative control.Lane 2 : Negative control siRNA from Qiagen was transfected.Lane 3 : siRNA for MYC was transfected.The data was provided by A. Khanna and Dr. J. Westermark of University of Tampere.