Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB600-302R - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-302R, RRID:AB_10004125
- Product name
- Mouse Monoclonal c-Myc Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.
- Reactivity
- Human, Mouse, Drosophila
- Host
- Mouse
- Conjugate
- Yellow dye
- Antigen sequence
Leucine zipper sequence (AEEQKLISEE
DLL)- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: c-Myc Antibody (9E10) [DyLight 550] [NB600-302R] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.