Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NB600-302C - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-302C, RRID:AB_10004129
- Product name
- Mouse Monoclonal c-Myc Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.
- Reactivity
- Human, Mouse, Drosophila
- Host
- Mouse
- Conjugate
- Red dye
- Antigen sequence
Leucine zipper sequence (AEEQKLISEE
DLL)- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
Submitted references Mitochondrial function provides instructive signals for activation-induced B-cell fates.
Jang KJ, Mano H, Aoki K, Hayashi T, Muto A, Nambu Y, Takahashi K, Itoh K, Taketani S, Nutt SL, Igarashi K, Shimizu A, Sugai M
Nature communications 2015 Apr 10;6:6750
Nature communications 2015 Apr 10;6:6750
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: c-Myc Antibody (9E10) [DyLight 650] [NB600-302C] - An intracellular stain was performed on HeLa cells with c-Myc Antibody [9E10] NB600-302C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.