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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- Ab097003 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant c-Myc Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human c-Myc Antibody, Recombinant, could be used for WB, IF, ICCand so on.Application:WB: 1/500-1/1000ICC/IF: 1/50-1/200Protein FunctionParticipates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 16-35- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,3 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant c-Myc Antibody (Ab097003) - Western Blot All lanes: Recombinant c-Myc Antibody (Ab097003) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 55 kDa Exposure time: 8.5 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant c-Myc Antibody (Ab097003) - IF/ICC Immunocytochemistry analysis of c-Myc (green) in HeLa using Recombinant c-Myc Antibody (Ab097003), and cells were counterstained with DAPI (blue).
