PA1-981

antibody from Invitrogen Antibodies

Targeting: MYC bHLHe39, c-Myc, MYCC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-981

Provider

Invitrogen Antibodies

Product name

Anti-Myc Tag Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-981 detects c-Myc tagged proteins. PA1-981 has been successfully used in WB and immunofluorescence procedures to detect the presence of expressed c-Myc tagged proteins in transfected mammalian cells. PA1-981 immunogen is a synthetic peptide corresponding to residues C E(410) Q K L I S E E D L(419) of human c-Myc. PA1-981 immunizing peptide (PEP-035) is available for use in neutralization and control experiments.

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Suppression and synthetic-lethal genetic relationships of ΔgpsB mutations indicate that GpsB mediates protein phosphorylation and penicillin-binding protein interactions in Streptococcus pneumoniae D39.

Rued BE, Zheng JJ, Mura A, Tsui HT, Boersma MJ, Mazny JL, Corona F, Perez AJ, Fadda D, Doubravová L, Buriánková K, Branny P, Massidda O, Winkler ME
Molecular microbiology 2017 Mar;103(6):931-957

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Mechanism of early light signaling by the carboxy-terminal output module of Arabidopsis phytochrome B.

Qiu Y, Pasoreck EK, Reddy AK, Nagatani A, Ma W, Chory J, Chen M
Nature communications 2017 Dec 4;8(1):1905

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Identification of Interferon-Stimulated Gene Proteins That Inhibit Human Parainfluenza Virus Type 3.

Rabbani MA, Ribaudo M, Guo JT, Barik S
Journal of virology 2016 Dec 15;90(24):11145-11156

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Unique nonstructural proteins of Pneumonia Virus of Mice (PVM) promote degradation of interferon (IFN) pathway components and IFN-stimulated gene proteins.

Dhar J, Barik S
Scientific reports 2016 Dec 1;6:38139

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Excessive sarcoplasmic/endoplasmic reticulum Ca2+-ATPase expression causes increased sarcoplasmic reticulum Ca2+ uptake but decreases myocyte shortening.

Teucher N, Prestle J, Seidler T, Currie S, Elliott EB, Reynolds DF, Schott P, Wagner S, Kogler H, Inesi G, Bers DM, Hasenfuss G, Smith GL
Circulation 2004 Dec 7;110(23):3553-9

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Increased expression of the glial glutamate transporter EAAT2 modulates excitotoxicity and delays the onset but not the outcome of ALS in mice.

Guo H, Lai L, Butchbach ME, Stockinger MP, Shan X, Bishop GA, Lin CL
Human molecular genetics 2003 Oct 1;12(19):2519-32

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of c-Myc was performed by loading 25 µg of 293t cells transfected with c-Myc -MYCBP (lane 1), transfected with c-Myc -FLI1 (lane 2) and transfected with 12Tag (lane 3) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a c-Myc Epitope Tag polyclonal antibody (Product # PA1-981) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). The image shows the target bands on c-Myc -MYCBP (19kDa), c-Myc -FLI1 (54kDa) and 12Tag (45kDa), which are consistent with predicted molecular weights for each target.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunolocalization of c-Myc in transfected mammalian cells using Product # PA1-981.