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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [30]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MA5-12077 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- c-Myc Monoclonal Antibody (9E10.3), Biotin
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA5-12077 targets c-myc in FACS, IF, IHC (P), IM, IP, and WB applications and shows reactivity with Human samples. The MA5-12077 immunogen is a synthetic peptide, aa 408-439, (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- 9E10.3
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references BCL2, BCL6, IGH, TP53, and MYC protein expression and gene rearrangements as prognostic markers in diffuse large B-cell lymphoma: a study of 44 Turkish patients.
EGFR and MYC gene copy number aberrations are more common in squamous cell carcinoma than keratoacanthoma: a FISH study.
The dichloromethane extract of the ethnomedicinal plant Neurolaena lobata inhibits NPM/ALK expression which is causal for anaplastic large cell lymphomagenesis.
MYC expression and distribution in normal mature lymphoid cells.
Expression of p53, Ki-67 and c-Myc proteins is predictive of the surgical molecular margin in colorectal carcinoma.
Nonconserved lysine residues attenuate the biological function of the low-risk human papillomavirus E7 protein.
Delaying Gal4-driven gene expression in the zebrafish with morpholinos and Gal80.
Detection of protein-RNA complexes in Xenopus oocytes.
Pro- and anticarcinogenic mechanisms of piceatannol are activated dose dependently in MCF-7 breast cancer cells.
Prognostic significance of oncogenic markers in ductal carcinoma in situ of the breast: a clinicopathologic study.
MYC insertions in diffuse-type gastric adenocarcinoma.
Proteomic analysis reveals Hrs ubiquitin-interacting motif-mediated ubiquitin signaling in multiple cellular processes.
Mitochondrial p32 is a critical mediator of ARF-induced apoptosis.
Molecular risk stratification of medulloblastoma patients based on immunohistochemical analysis of MYC, LDHB, and CCNB1 expression.
Mutational activation of ErbB2 reveals a new protein kinase autoinhibition mechanism.
Expression and prognostic implications of apoptosis-related proteins in locally unresectable non-small cell lung cancers.
The shed ectodomain of type XIII collagen associates with the fibrillar fibronectin matrix and may interfere with its assembly in vitro.
Defined genetic events associated with the spontaneous in vitro transformation of ElA/Ras-expressing human IMR90 fibroblasts.
Downregulation of EGF receptor signaling in pancreatic islets causes diabetes due to impaired postnatal beta-cell growth.
Transmembrane peptides as inhibitors of ErbB receptor signaling.
Sue1p is required for degradation of labile forms of altered cytochromes C in yeast mitochondria.
Upstream stimulatory factor but not c-Myc enhances transcription of the human polymeric immunoglobulin receptor gene.
Upstream stimulatory factor but not c-Myc enhances transcription of the human polymeric immunoglobulin receptor gene.
Metastasis-associated protein 1 interacts with NRIF3, an estrogen-inducible nuclear receptor coregulator.
Construction of tumor-specific cells expressing a membrane-anchored single-chain Fv of anti-ErbB-2 antibody.
Construction of tumor-specific cells expressing a membrane-anchored single-chain Fv of anti-ErbB-2 antibody.
Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53.
Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53.
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways.
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways.
Akay OM, Aras BD, Isiksoy S, Toprak C, Mutlu FS, Artan S, Oner U, Gulbas Z
Cancer genetics 2014 Mar;207(3):87-93
Cancer genetics 2014 Mar;207(3):87-93
EGFR and MYC gene copy number aberrations are more common in squamous cell carcinoma than keratoacanthoma: a FISH study.
Jacobs MS, Persons DL, Fraga GR
Journal of cutaneous pathology 2013 May;40(5):447-54
Journal of cutaneous pathology 2013 May;40(5):447-54
The dichloromethane extract of the ethnomedicinal plant Neurolaena lobata inhibits NPM/ALK expression which is causal for anaplastic large cell lymphomagenesis.
Unger C, Popescu R, Giessrigl B, Laimer D, Heider S, Seelinger M, Diaz R, Wallnöfer B, Egger G, Hassler M, Knöfler M, Saleh L, Sahin E, Grusch M, Fritzer-Szekeres M, Dolznig H, Frisch R, Kenner L, Kopp B, Krupitza G
International journal of oncology 2013 Jan;42(1):338-48
International journal of oncology 2013 Jan;42(1):338-48
MYC expression and distribution in normal mature lymphoid cells.
Cattoretti G
The Journal of pathology 2013 Feb;229(3):430-40
The Journal of pathology 2013 Feb;229(3):430-40
Expression of p53, Ki-67 and c-Myc proteins is predictive of the surgical molecular margin in colorectal carcinoma.
Li N, Wang J, Shen S, Bu X, Tian X, Huang P
Pathology oncology research : POR 2011 Sep;17(3):479-87
Pathology oncology research : POR 2011 Sep;17(3):479-87
Nonconserved lysine residues attenuate the biological function of the low-risk human papillomavirus E7 protein.
Genovese NJ, Broker TR, Chow LT
Journal of virology 2011 Jun;85(11):5546-54
Journal of virology 2011 Jun;85(11):5546-54
Delaying Gal4-driven gene expression in the zebrafish with morpholinos and Gal80.
Faucherre A, López-Schier H
PloS one 2011 Jan 26;6(1):e16587
PloS one 2011 Jan 26;6(1):e16587
Detection of protein-RNA complexes in Xenopus oocytes.
Huber PW, Zhao WM
Methods (San Diego, Calif.) 2010 May;51(1):82-6
Methods (San Diego, Calif.) 2010 May;51(1):82-6
Pro- and anticarcinogenic mechanisms of piceatannol are activated dose dependently in MCF-7 breast cancer cells.
Vo NT, Madlener S, Bago-Horvath Z, Herbacek I, Stark N, Gridling M, Probst P, Giessrigl B, Bauer S, Vonach C, Saiko P, Grusch M, Szekeres T, Fritzer-Szekeres M, Jäger W, Krupitza G, Soleiman A
Carcinogenesis 2010 Dec;31(12):2074-81
Carcinogenesis 2010 Dec;31(12):2074-81
Prognostic significance of oncogenic markers in ductal carcinoma in situ of the breast: a clinicopathologic study.
Altintas S, Lambein K, Huizing MT, Braems G, Asjoe FT, Hellemans H, Van Marck E, Weyler J, Praet M, Van den Broecke R, Vermorken JB, Tjalma WA
The breast journal 2009 Mar-Apr;15(2):120-32
The breast journal 2009 Mar-Apr;15(2):120-32
MYC insertions in diffuse-type gastric adenocarcinoma.
Calcagno DQ, Guimarães AC, Leal MF, Seabra AD, Khayat AS, Pontes TB, Assumpção PP, De Arruda Cardoso Smith M, Burbano RR
Anticancer research 2009 Jul;29(7):2479-83
Anticancer research 2009 Jul;29(7):2479-83
Proteomic analysis reveals Hrs ubiquitin-interacting motif-mediated ubiquitin signaling in multiple cellular processes.
Pridgeon JW, Webber EA, Sha D, Li L, Chin LS
The FEBS journal 2009 Jan;276(1):118-31
The FEBS journal 2009 Jan;276(1):118-31
Mitochondrial p32 is a critical mediator of ARF-induced apoptosis.
Itahana K, Zhang Y
Cancer cell 2008 Jun;13(6):542-53
Cancer cell 2008 Jun;13(6):542-53
Molecular risk stratification of medulloblastoma patients based on immunohistochemical analysis of MYC, LDHB, and CCNB1 expression.
de Haas T, Hasselt N, Troost D, Caron H, Popovic M, Zadravec-Zaletel L, Grajkowska W, Perek M, Osterheld MC, Ellison D, Baas F, Versteeg R, Kool M
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Jul 1;14(13):4154-60
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Jul 1;14(13):4154-60
Mutational activation of ErbB2 reveals a new protein kinase autoinhibition mechanism.
Fan YX, Wong L, Ding J, Spiridonov NA, Johnson RC, Johnson GR
The Journal of biological chemistry 2008 Jan 18;283(3):1588-96
The Journal of biological chemistry 2008 Jan 18;283(3):1588-96
Expression and prognostic implications of apoptosis-related proteins in locally unresectable non-small cell lung cancers.
Fokkema E, Timens W, de Vries EG, de Jong S, Fidler V, Meijer C, Groen HJ
Lung cancer (Amsterdam, Netherlands) 2006 May;52(2):241-7
Lung cancer (Amsterdam, Netherlands) 2006 May;52(2):241-7
The shed ectodomain of type XIII collagen associates with the fibrillar fibronectin matrix and may interfere with its assembly in vitro.
Väisänen MR, Väisänen T, Tu H, Pirilä P, Sormunen R, Pihlajaniemi T
The Biochemical journal 2006 Jan 1;393(Pt 1):43-50
The Biochemical journal 2006 Jan 1;393(Pt 1):43-50
Defined genetic events associated with the spontaneous in vitro transformation of ElA/Ras-expressing human IMR90 fibroblasts.
Mason DX, Keppler D, Zhang J, Jackson TJ, Seger YR, Matsui S, Abreo F, Cowell JK, Hannon GJ, Lowe SW, Lin AW
Carcinogenesis 2006 Feb;27(2):350-9
Carcinogenesis 2006 Feb;27(2):350-9
Downregulation of EGF receptor signaling in pancreatic islets causes diabetes due to impaired postnatal beta-cell growth.
Miettinen PJ, Ustinov J, Ormio P, Gao R, Palgi J, Hakonen E, Juntti-Berggren L, Berggren PO, Otonkoski T
Diabetes 2006 Dec;55(12):3299-308
Diabetes 2006 Dec;55(12):3299-308
Transmembrane peptides as inhibitors of ErbB receptor signaling.
Bennasroune A, Fickova M, Gardin A, Dirrig-Grosch S, Aunis D, Crémel G, Hubert P
Molecular biology of the cell 2004 Jul;15(7):3464-74
Molecular biology of the cell 2004 Jul;15(7):3464-74
Sue1p is required for degradation of labile forms of altered cytochromes C in yeast mitochondria.
Wei J, Sherman F
The Journal of biological chemistry 2004 Jul 16;279(29):30449-58
The Journal of biological chemistry 2004 Jul 16;279(29):30449-58
Upstream stimulatory factor but not c-Myc enhances transcription of the human polymeric immunoglobulin receptor gene.
Bruno ME, West RB, Schneeman TA, Bresnick EH, Kaetzel CS
Molecular immunology 2004 Jan;40(10):695-708
Molecular immunology 2004 Jan;40(10):695-708
Upstream stimulatory factor but not c-Myc enhances transcription of the human polymeric immunoglobulin receptor gene.
Bruno ME, West RB, Schneeman TA, Bresnick EH, Kaetzel CS
Molecular immunology 2004 Jan;40(10):695-708
Molecular immunology 2004 Jan;40(10):695-708
Metastasis-associated protein 1 interacts with NRIF3, an estrogen-inducible nuclear receptor coregulator.
Talukder AH, Gururaj A, Mishra SK, Vadlamudi RK, Kumar R
Molecular and cellular biology 2004 Aug;24(15):6581-91
Molecular and cellular biology 2004 Aug;24(15):6581-91
Construction of tumor-specific cells expressing a membrane-anchored single-chain Fv of anti-ErbB-2 antibody.
Suzuki M, Shinkai M, Honda H, Kamihira M, Iijima S, Kobayashi T
Biochimica et biophysica acta 2001 Feb 16;1525(1-2):191-6
Biochimica et biophysica acta 2001 Feb 16;1525(1-2):191-6
Construction of tumor-specific cells expressing a membrane-anchored single-chain Fv of anti-ErbB-2 antibody.
Suzuki M, Shinkai M, Honda H, Kamihira M, Iijima S, Kobayashi T
Biochimica et biophysica acta 2001 Feb 16;1525(1-2):191-6
Biochimica et biophysica acta 2001 Feb 16;1525(1-2):191-6
Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53.
Zhang Y, Xiong Y
Molecular cell 1999 May;3(5):579-91
Molecular cell 1999 May;3(5):579-91
Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53.
Zhang Y, Xiong Y
Molecular cell 1999 May;3(5):579-91
Molecular cell 1999 May;3(5):579-91
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways.
Zhang Y, Xiong Y, Yarbrough WG
Cell 1998 Mar 20;92(6):725-34
Cell 1998 Mar 20;92(6):725-34
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways.
Zhang Y, Xiong Y, Yarbrough WG
Cell 1998 Mar 20;92(6):725-34
Cell 1998 Mar 20;92(6):725-34
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of c-myc using c-myc Monoclonal Antibody (Product # MA5-12077) on Raji Cells.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-c-Myc Monoclonal Antibody (9E10.3), Biotin (Product # MA5-12077) by loading whole cell extracts of untransfected and transiently transfected HEK-293 lysates: untransfected, 40 µg (Lane 1), empty vector control, 40 µg (Lane 2), Myc-p65-V5, 40 µg (Lane 3), Myc-p65-V5, 20 µg (Lane 4), p65-HA, 40 µg (Lane 5) and 25 ng of Positope (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). A ~65 kDa band corresponding to Myc-p65-V5 was observed in HEK293 transfected lysates on probing with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Poly-HRP Streptavidin (Product # N200, 1:4000 dilution). Positope (Product # R90050) is a 53 kDa recombinant protein consisting of multiple epitope tags but lacks HA tag, which has been used as a positive control for c-Myc tag detection. No cross-reactivity was seen with HA-tagged p65 expressing lysate.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human breast carcinoma stained with c-myc antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.
- Conjugate
- Biotin
