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- Antibody Data
- Antigen structure
- References [65]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- MA5-12080 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- c-Myc Monoclonal Antibody (9E10.3)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA5-12080 detects-myc in Human samples Does not react with mouse, rat and chicken. Others not tested..
- Antibody clone number
- 9E10.3
- Concentration
- 0.2 mg/mL
Submitted references Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis.
Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis.
CLCA2 Interactor EVA1 Is Required for Mammary Epithelial Cell Differentiation.
YWHAE silencing induces cell proliferation, invasion and migration through the up-regulation of CDC25B and MYC in gastric cancer cells: new insights about YWHAE role in the tumor development and metastasis process.
Human Cytomegalovirus pUL93 Links Nucleocapsid Maturation and Nuclear Egress.
BCL2, BCL6, IGH, TP53, and MYC protein expression and gene rearrangements as prognostic markers in diffuse large B-cell lymphoma: a study of 44 Turkish patients.
EGFR and MYC gene copy number aberrations are more common in squamous cell carcinoma than keratoacanthoma: a FISH study.
The dichloromethane extract of the ethnomedicinal plant Neurolaena lobata inhibits NPM/ALK expression which is causal for anaplastic large cell lymphomagenesis.
MYC expression and distribution in normal mature lymphoid cells.
Circulating hepatitis B surface antigen particles carry hepatocellular microRNAs.
Expression of peroxisome proliferator activated receptor-gamma (PPAR-γ) in human non-small cell lung carcinoma: correlation with clinicopathological parameters, proliferation and apoptosis related molecules and patients' survival.
Activation of myeloid cell-specific adhesion class G protein-coupled receptor EMR2 via ligation-induced translocation and interaction of receptor subunits in lipid raft microdomains.
A dominant negative zebrafish Ahr2 partially protects developing zebrafish from dioxin toxicity.
Expression of p53, Ki-67 and c-Myc proteins is predictive of the surgical molecular margin in colorectal carcinoma.
Ribosomal protein L11 recruits miR-24/miRISC to repress c-Myc expression in response to ribosomal stress.
Nonconserved lysine residues attenuate the biological function of the low-risk human papillomavirus E7 protein.
Delaying Gal4-driven gene expression in the zebrafish with morpholinos and Gal80.
Detection of protein-RNA complexes in Xenopus oocytes.
Pro- and anticarcinogenic mechanisms of piceatannol are activated dose dependently in MCF-7 breast cancer cells.
Cytoplasmic domain of human Fcalpha/mu receptor is required for ligand internalization.
Prognostic significance of oncogenic markers in ductal carcinoma in situ of the breast: a clinicopathologic study.
NFX1-123 increases hTERT expression and telomerase activity posttranscriptionally in human papillomavirus type 16 E6 keratinocytes.
MYC insertions in diffuse-type gastric adenocarcinoma.
Proteomic analysis reveals Hrs ubiquitin-interacting motif-mediated ubiquitin signaling in multiple cellular processes.
Prognostic significance of c-Met expression in glioblastomas.
Mitochondrial p32 is a critical mediator of ARF-induced apoptosis.
Molecular risk stratification of medulloblastoma patients based on immunohistochemical analysis of MYC, LDHB, and CCNB1 expression.
Mutational activation of ErbB2 reveals a new protein kinase autoinhibition mechanism.
Expression and prognostic implications of apoptosis-related proteins in locally unresectable non-small cell lung cancers.
Human melanocortin receptor 2 expression and functionality: effects of protein kinase A and protein kinase C on desensitization and internalization.
The shed ectodomain of type XIII collagen associates with the fibrillar fibronectin matrix and may interfere with its assembly in vitro.
Defined genetic events associated with the spontaneous in vitro transformation of ElA/Ras-expressing human IMR90 fibroblasts.
Downregulation of EGF receptor signaling in pancreatic islets causes diabetes due to impaired postnatal beta-cell growth.
c-Myc-regulated microRNAs modulate E2F1 expression.
Transmembrane peptides as inhibitors of ErbB receptor signaling.
Sue1p is required for degradation of labile forms of altered cytochromes C in yeast mitochondria.
Upstream stimulatory factor but not c-Myc enhances transcription of the human polymeric immunoglobulin receptor gene.
Upstream stimulatory factor but not c-Myc enhances transcription of the human polymeric immunoglobulin receptor gene.
Metastasis-associated protein 1 interacts with NRIF3, an estrogen-inducible nuclear receptor coregulator.
A core function for p120-catenin in cadherin turnover.
AMP-activated protein kinase can induce apoptosis of insulin-producing MIN6 cells through stimulation of c-Jun-N-terminal kinase.
AMP-activated protein kinase can induce apoptosis of insulin-producing MIN6 cells through stimulation of c-Jun-N-terminal kinase.
Staring, a novel E3 ubiquitin-protein ligase that targets syntaxin 1 for degradation.
A mutation in the beta interaction domain of the Ca(2+) channel alpha(1C) subunit reduces the affinity of the (+)-[(3)H]isradipine binding site.
A mutation in the beta interaction domain of the Ca(2+) channel alpha(1C) subunit reduces the affinity of the (+)-[(3)H]isradipine binding site.
Quantitative measurement of anti-ErbB-2 antibody by flow cytometry and ELISA.
Quantitative measurement of anti-ErbB-2 antibody by flow cytometry and ELISA.
GRP94 (gp96) and GRP94 N-terminal geldanamycin binding domain elicit tissue nonrestricted tumor suppression.
GRP94 (gp96) and GRP94 N-terminal geldanamycin binding domain elicit tissue nonrestricted tumor suppression.
Addition of a c-myc epitope tag within the VEGF protein does not affect in vitro biological activity.
The fused protein kinase regulates Hedgehog-stimulated transcriptional activation in Drosophila Schneider 2 cells.
PI3-kinase in concert with Src promotes the S-phase entry of oestradiol-stimulated MCF-7 cells.
Construction of tumor-specific cells expressing a membrane-anchored single-chain Fv of anti-ErbB-2 antibody.
Construction of tumor-specific cells expressing a membrane-anchored single-chain Fv of anti-ErbB-2 antibody.
Early and persistent bone marrow hematopoiesis defect in simian/human immunodeficiency virus-infected macaques despite efficient reduction of viremia by highly active antiretroviral therapy during primary infection.
Opposite effects of antimicrotubule agents on c-myc oncogene expression depending on the cell lines used.
Opposite effects of antimicrotubule agents on c-myc oncogene expression depending on the cell lines used.
The Smad4 activation domain (SAD) is a proline-rich, p300-dependent transcriptional activation domain.
The Smad4 activation domain (SAD) is a proline-rich, p300-dependent transcriptional activation domain.
Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53.
Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53.
Truncated N-terminal fragments of huntingtin with expanded glutamine repeats form nuclear and cytoplasmic aggregates in cell culture.
Truncated N-terminal fragments of huntingtin with expanded glutamine repeats form nuclear and cytoplasmic aggregates in cell culture.
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways.
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways.
Wang R, Peng C, Song J, Hua Y, Wu Q, Deng L, Cao Y, Zhang J, Zhang L, Wu L, Hou L
International journal of oncology 2022 Mar;60(3)
International journal of oncology 2022 Mar;60(3)
Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis.
Macia MS, Halbritter J, Delous M, Bredrup C, Gutter A, Filhol E, Mellgren AEC, Leh S, Bizet A, Braun DA, Gee HY, Silbermann F, Henry C, Krug P, Bole-Feysot C, Nitschké P, Joly D, Nicoud P, Paget A, Haugland H, Brackmann D, Ahmet N, Sandford R, Cengiz N, Knappskog PM, Boman H, Linghu B, Yang F, Oakeley EJ, Saint Mézard P, Sailer AW, Johansson S, Rødahl E, Saunier S, Hildebrandt F, Benmerah A
American journal of human genetics 2017 Feb 2;100(2):323-333
American journal of human genetics 2017 Feb 2;100(2):323-333
CLCA2 Interactor EVA1 Is Required for Mammary Epithelial Cell Differentiation.
Ramena G, Yin Y, Yu Y, Walia V, Elble RC
PloS one 2016;11(3):e0147489
PloS one 2016;11(3):e0147489
YWHAE silencing induces cell proliferation, invasion and migration through the up-regulation of CDC25B and MYC in gastric cancer cells: new insights about YWHAE role in the tumor development and metastasis process.
Leal MF, Ribeiro HF, Rey JA, Pinto GR, Smith MC, Moreira-Nunes CA, Assumpção PP, Lamarão LM, Calcagno DQ, Montenegro RC, Burbano RR
Oncotarget 2016 Dec 20;7(51):85393-85410
Oncotarget 2016 Dec 20;7(51):85393-85410
Human Cytomegalovirus pUL93 Links Nucleocapsid Maturation and Nuclear Egress.
DeRussy BM, Boland MT, Tandon R
Journal of virology 2016 Aug 15;90(16):7109-7117
Journal of virology 2016 Aug 15;90(16):7109-7117
BCL2, BCL6, IGH, TP53, and MYC protein expression and gene rearrangements as prognostic markers in diffuse large B-cell lymphoma: a study of 44 Turkish patients.
Akay OM, Aras BD, Isiksoy S, Toprak C, Mutlu FS, Artan S, Oner U, Gulbas Z
Cancer genetics 2014 Mar;207(3):87-93
Cancer genetics 2014 Mar;207(3):87-93
EGFR and MYC gene copy number aberrations are more common in squamous cell carcinoma than keratoacanthoma: a FISH study.
Jacobs MS, Persons DL, Fraga GR
Journal of cutaneous pathology 2013 May;40(5):447-54
Journal of cutaneous pathology 2013 May;40(5):447-54
The dichloromethane extract of the ethnomedicinal plant Neurolaena lobata inhibits NPM/ALK expression which is causal for anaplastic large cell lymphomagenesis.
Unger C, Popescu R, Giessrigl B, Laimer D, Heider S, Seelinger M, Diaz R, Wallnöfer B, Egger G, Hassler M, Knöfler M, Saleh L, Sahin E, Grusch M, Fritzer-Szekeres M, Dolznig H, Frisch R, Kenner L, Kopp B, Krupitza G
International journal of oncology 2013 Jan;42(1):338-48
International journal of oncology 2013 Jan;42(1):338-48
MYC expression and distribution in normal mature lymphoid cells.
Cattoretti G
The Journal of pathology 2013 Feb;229(3):430-40
The Journal of pathology 2013 Feb;229(3):430-40
Circulating hepatitis B surface antigen particles carry hepatocellular microRNAs.
Novellino L, Rossi RL, Bonino F, Cavallone D, Abrignani S, Pagani M, Brunetto MR
PloS one 2012;7(3):e31952
PloS one 2012;7(3):e31952
Expression of peroxisome proliferator activated receptor-gamma (PPAR-γ) in human non-small cell lung carcinoma: correlation with clinicopathological parameters, proliferation and apoptosis related molecules and patients' survival.
Giaginis C, Politi E, Alexandrou P, Sfiniadakis J, Kouraklis G, Theocharis S
Pathology oncology research : POR 2012 Oct;18(4):875-83
Pathology oncology research : POR 2012 Oct;18(4):875-83
Activation of myeloid cell-specific adhesion class G protein-coupled receptor EMR2 via ligation-induced translocation and interaction of receptor subunits in lipid raft microdomains.
Huang YS, Chiang NY, Hu CH, Hsiao CC, Cheng KF, Tsai WP, Yona S, Stacey M, Gordon S, Chang GW, Lin HH
Molecular and cellular biology 2012 Apr;32(8):1408-20
Molecular and cellular biology 2012 Apr;32(8):1408-20
A dominant negative zebrafish Ahr2 partially protects developing zebrafish from dioxin toxicity.
Lanham KA, Prasch AL, Weina KM, Peterson RE, Heideman W
PloS one 2011;6(12):e28020
PloS one 2011;6(12):e28020
Expression of p53, Ki-67 and c-Myc proteins is predictive of the surgical molecular margin in colorectal carcinoma.
Li N, Wang J, Shen S, Bu X, Tian X, Huang P
Pathology oncology research : POR 2011 Sep;17(3):479-87
Pathology oncology research : POR 2011 Sep;17(3):479-87
Ribosomal protein L11 recruits miR-24/miRISC to repress c-Myc expression in response to ribosomal stress.
Challagundla KB, Sun XX, Zhang X, DeVine T, Zhang Q, Sears RC, Dai MS
Molecular and cellular biology 2011 Oct;31(19):4007-21
Molecular and cellular biology 2011 Oct;31(19):4007-21
Nonconserved lysine residues attenuate the biological function of the low-risk human papillomavirus E7 protein.
Genovese NJ, Broker TR, Chow LT
Journal of virology 2011 Jun;85(11):5546-54
Journal of virology 2011 Jun;85(11):5546-54
Delaying Gal4-driven gene expression in the zebrafish with morpholinos and Gal80.
Faucherre A, López-Schier H
PloS one 2011 Jan 26;6(1):e16587
PloS one 2011 Jan 26;6(1):e16587
Detection of protein-RNA complexes in Xenopus oocytes.
Huber PW, Zhao WM
Methods (San Diego, Calif.) 2010 May;51(1):82-6
Methods (San Diego, Calif.) 2010 May;51(1):82-6
Pro- and anticarcinogenic mechanisms of piceatannol are activated dose dependently in MCF-7 breast cancer cells.
Vo NT, Madlener S, Bago-Horvath Z, Herbacek I, Stark N, Gridling M, Probst P, Giessrigl B, Bauer S, Vonach C, Saiko P, Grusch M, Szekeres T, Fritzer-Szekeres M, Jäger W, Krupitza G, Soleiman A
Carcinogenesis 2010 Dec;31(12):2074-81
Carcinogenesis 2010 Dec;31(12):2074-81
Cytoplasmic domain of human Fcalpha/mu receptor is required for ligand internalization.
Yang L, Shen L, Shao Y, Zhao Q, Zhang W
Cellular immunology 2009;258(1):78-82
Cellular immunology 2009;258(1):78-82
Prognostic significance of oncogenic markers in ductal carcinoma in situ of the breast: a clinicopathologic study.
Altintas S, Lambein K, Huizing MT, Braems G, Asjoe FT, Hellemans H, Van Marck E, Weyler J, Praet M, Van den Broecke R, Vermorken JB, Tjalma WA
The breast journal 2009 Mar-Apr;15(2):120-32
The breast journal 2009 Mar-Apr;15(2):120-32
NFX1-123 increases hTERT expression and telomerase activity posttranscriptionally in human papillomavirus type 16 E6 keratinocytes.
Katzenellenbogen RA, Vliet-Gregg P, Xu M, Galloway DA
Journal of virology 2009 Jul;83(13):6446-56
Journal of virology 2009 Jul;83(13):6446-56
MYC insertions in diffuse-type gastric adenocarcinoma.
Calcagno DQ, Guimarães AC, Leal MF, Seabra AD, Khayat AS, Pontes TB, Assumpção PP, De Arruda Cardoso Smith M, Burbano RR
Anticancer research 2009 Jul;29(7):2479-83
Anticancer research 2009 Jul;29(7):2479-83
Proteomic analysis reveals Hrs ubiquitin-interacting motif-mediated ubiquitin signaling in multiple cellular processes.
Pridgeon JW, Webber EA, Sha D, Li L, Chin LS
The FEBS journal 2009 Jan;276(1):118-31
The FEBS journal 2009 Jan;276(1):118-31
Prognostic significance of c-Met expression in glioblastomas.
Kong DS, Song SY, Kim DH, Joo KM, Yoo JS, Koh JS, Dong SM, Suh YL, Lee JI, Park K, Kim JH, Nam DH
Cancer 2009 Jan 1;115(1):140-8
Cancer 2009 Jan 1;115(1):140-8
Mitochondrial p32 is a critical mediator of ARF-induced apoptosis.
Itahana K, Zhang Y
Cancer cell 2008 Jun;13(6):542-53
Cancer cell 2008 Jun;13(6):542-53
Molecular risk stratification of medulloblastoma patients based on immunohistochemical analysis of MYC, LDHB, and CCNB1 expression.
de Haas T, Hasselt N, Troost D, Caron H, Popovic M, Zadravec-Zaletel L, Grajkowska W, Perek M, Osterheld MC, Ellison D, Baas F, Versteeg R, Kool M
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Jul 1;14(13):4154-60
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Jul 1;14(13):4154-60
Mutational activation of ErbB2 reveals a new protein kinase autoinhibition mechanism.
Fan YX, Wong L, Ding J, Spiridonov NA, Johnson RC, Johnson GR
The Journal of biological chemistry 2008 Jan 18;283(3):1588-96
The Journal of biological chemistry 2008 Jan 18;283(3):1588-96
Expression and prognostic implications of apoptosis-related proteins in locally unresectable non-small cell lung cancers.
Fokkema E, Timens W, de Vries EG, de Jong S, Fidler V, Meijer C, Groen HJ
Lung cancer (Amsterdam, Netherlands) 2006 May;52(2):241-7
Lung cancer (Amsterdam, Netherlands) 2006 May;52(2):241-7
Human melanocortin receptor 2 expression and functionality: effects of protein kinase A and protein kinase C on desensitization and internalization.
Kilianova Z, Basora N, Kilian P, Payet MD, Gallo-Payet N
Endocrinology 2006 May;147(5):2325-37
Endocrinology 2006 May;147(5):2325-37
The shed ectodomain of type XIII collagen associates with the fibrillar fibronectin matrix and may interfere with its assembly in vitro.
Väisänen MR, Väisänen T, Tu H, Pirilä P, Sormunen R, Pihlajaniemi T
The Biochemical journal 2006 Jan 1;393(Pt 1):43-50
The Biochemical journal 2006 Jan 1;393(Pt 1):43-50
Defined genetic events associated with the spontaneous in vitro transformation of ElA/Ras-expressing human IMR90 fibroblasts.
Mason DX, Keppler D, Zhang J, Jackson TJ, Seger YR, Matsui S, Abreo F, Cowell JK, Hannon GJ, Lowe SW, Lin AW
Carcinogenesis 2006 Feb;27(2):350-9
Carcinogenesis 2006 Feb;27(2):350-9
Downregulation of EGF receptor signaling in pancreatic islets causes diabetes due to impaired postnatal beta-cell growth.
Miettinen PJ, Ustinov J, Ormio P, Gao R, Palgi J, Hakonen E, Juntti-Berggren L, Berggren PO, Otonkoski T
Diabetes 2006 Dec;55(12):3299-308
Diabetes 2006 Dec;55(12):3299-308
c-Myc-regulated microRNAs modulate E2F1 expression.
O'Donnell KA, Wentzel EA, Zeller KI, Dang CV, Mendell JT
Nature 2005 Jun 9;435(7043):839-43
Nature 2005 Jun 9;435(7043):839-43
Transmembrane peptides as inhibitors of ErbB receptor signaling.
Bennasroune A, Fickova M, Gardin A, Dirrig-Grosch S, Aunis D, Crémel G, Hubert P
Molecular biology of the cell 2004 Jul;15(7):3464-74
Molecular biology of the cell 2004 Jul;15(7):3464-74
Sue1p is required for degradation of labile forms of altered cytochromes C in yeast mitochondria.
Wei J, Sherman F
The Journal of biological chemistry 2004 Jul 16;279(29):30449-58
The Journal of biological chemistry 2004 Jul 16;279(29):30449-58
Upstream stimulatory factor but not c-Myc enhances transcription of the human polymeric immunoglobulin receptor gene.
Bruno ME, West RB, Schneeman TA, Bresnick EH, Kaetzel CS
Molecular immunology 2004 Jan;40(10):695-708
Molecular immunology 2004 Jan;40(10):695-708
Upstream stimulatory factor but not c-Myc enhances transcription of the human polymeric immunoglobulin receptor gene.
Bruno ME, West RB, Schneeman TA, Bresnick EH, Kaetzel CS
Molecular immunology 2004 Jan;40(10):695-708
Molecular immunology 2004 Jan;40(10):695-708
Metastasis-associated protein 1 interacts with NRIF3, an estrogen-inducible nuclear receptor coregulator.
Talukder AH, Gururaj A, Mishra SK, Vadlamudi RK, Kumar R
Molecular and cellular biology 2004 Aug;24(15):6581-91
Molecular and cellular biology 2004 Aug;24(15):6581-91
A core function for p120-catenin in cadherin turnover.
Davis MA, Ireton RC, Reynolds AB
The Journal of cell biology 2003 Nov 10;163(3):525-34
The Journal of cell biology 2003 Nov 10;163(3):525-34
AMP-activated protein kinase can induce apoptosis of insulin-producing MIN6 cells through stimulation of c-Jun-N-terminal kinase.
Kefas BA, Cai Y, Ling Z, Heimberg H, Hue L, Pipeleers D, Van de Casteele M
Journal of molecular endocrinology 2003 Apr;30(2):151-61
Journal of molecular endocrinology 2003 Apr;30(2):151-61
AMP-activated protein kinase can induce apoptosis of insulin-producing MIN6 cells through stimulation of c-Jun-N-terminal kinase.
Kefas BA, Cai Y, Ling Z, Heimberg H, Hue L, Pipeleers D, Van de Casteele M
Journal of molecular endocrinology 2003 Apr;30(2):151-61
Journal of molecular endocrinology 2003 Apr;30(2):151-61
Staring, a novel E3 ubiquitin-protein ligase that targets syntaxin 1 for degradation.
Chin LS, Vavalle JP, Li L
The Journal of biological chemistry 2002 Sep 20;277(38):35071-9
The Journal of biological chemistry 2002 Sep 20;277(38):35071-9
A mutation in the beta interaction domain of the Ca(2+) channel alpha(1C) subunit reduces the affinity of the (+)-[(3)H]isradipine binding site.
Hitzl M, Striessnig J, Neuhuber B, Flucher BE
FEBS letters 2002 Jul 31;524(1-3):188-92
FEBS letters 2002 Jul 31;524(1-3):188-92
A mutation in the beta interaction domain of the Ca(2+) channel alpha(1C) subunit reduces the affinity of the (+)-[(3)H]isradipine binding site.
Hitzl M, Striessnig J, Neuhuber B, Flucher BE
FEBS letters 2002 Jul 31;524(1-3):188-92
FEBS letters 2002 Jul 31;524(1-3):188-92
Quantitative measurement of anti-ErbB-2 antibody by flow cytometry and ELISA.
Piechocki MP, Pilon SA, Wei WZ
Journal of immunological methods 2002 Jan 1;259(1-2):33-42
Journal of immunological methods 2002 Jan 1;259(1-2):33-42
Quantitative measurement of anti-ErbB-2 antibody by flow cytometry and ELISA.
Piechocki MP, Pilon SA, Wei WZ
Journal of immunological methods 2002 Jan 1;259(1-2):33-42
Journal of immunological methods 2002 Jan 1;259(1-2):33-42
GRP94 (gp96) and GRP94 N-terminal geldanamycin binding domain elicit tissue nonrestricted tumor suppression.
Baker-LePain JC, Sarzotti M, Fields TA, Li CY, Nicchitta CV
The Journal of experimental medicine 2002 Dec 2;196(11):1447-59
The Journal of experimental medicine 2002 Dec 2;196(11):1447-59
GRP94 (gp96) and GRP94 N-terminal geldanamycin binding domain elicit tissue nonrestricted tumor suppression.
Baker-LePain JC, Sarzotti M, Fields TA, Li CY, Nicchitta CV
The Journal of experimental medicine 2002 Dec 2;196(11):1447-59
The Journal of experimental medicine 2002 Dec 2;196(11):1447-59
Addition of a c-myc epitope tag within the VEGF protein does not affect in vitro biological activity.
Chavand O, Spilsbury K, Rakoczy PE
Biochemistry and cell biology = Biochimie et biologie cellulaire 2001;79(1):107-12
Biochemistry and cell biology = Biochimie et biologie cellulaire 2001;79(1):107-12
The fused protein kinase regulates Hedgehog-stimulated transcriptional activation in Drosophila Schneider 2 cells.
Fukumoto T, Watanabe-Fukunaga R, Fujisawa K, Nagata S, Fukunaga R
The Journal of biological chemistry 2001 Oct 19;276(42):38441-8
The Journal of biological chemistry 2001 Oct 19;276(42):38441-8
PI3-kinase in concert with Src promotes the S-phase entry of oestradiol-stimulated MCF-7 cells.
Castoria G, Migliaccio A, Bilancio A, Di Domenico M, de Falco A, Lombardi M, Fiorentino R, Varricchio L, Barone MV, Auricchio F
The EMBO journal 2001 Nov 1;20(21):6050-9
The EMBO journal 2001 Nov 1;20(21):6050-9
Construction of tumor-specific cells expressing a membrane-anchored single-chain Fv of anti-ErbB-2 antibody.
Suzuki M, Shinkai M, Honda H, Kamihira M, Iijima S, Kobayashi T
Biochimica et biophysica acta 2001 Feb 16;1525(1-2):191-6
Biochimica et biophysica acta 2001 Feb 16;1525(1-2):191-6
Construction of tumor-specific cells expressing a membrane-anchored single-chain Fv of anti-ErbB-2 antibody.
Suzuki M, Shinkai M, Honda H, Kamihira M, Iijima S, Kobayashi T
Biochimica et biophysica acta 2001 Feb 16;1525(1-2):191-6
Biochimica et biophysica acta 2001 Feb 16;1525(1-2):191-6
Early and persistent bone marrow hematopoiesis defect in simian/human immunodeficiency virus-infected macaques despite efficient reduction of viremia by highly active antiretroviral therapy during primary infection.
Thiebot H, Louache F, Vaslin B, de Revel T, Neildez O, Larghero J, Vainchenker W, Dormont D, Le Grand R
Journal of virology 2001 Dec;75(23):11594-602
Journal of virology 2001 Dec;75(23):11594-602
Opposite effects of antimicrotubule agents on c-myc oncogene expression depending on the cell lines used.
Bourgarel-Rey V, El Khyari S, Rimet O, Bordas B, Guigal N, Braguer D, Seree E, Barra Y, Briand C
European journal of cancer (Oxford, England : 1990) 2000 May;36(8):1043-9
European journal of cancer (Oxford, England : 1990) 2000 May;36(8):1043-9
Opposite effects of antimicrotubule agents on c-myc oncogene expression depending on the cell lines used.
Bourgarel-Rey V, El Khyari S, Rimet O, Bordas B, Guigal N, Braguer D, Seree E, Barra Y, Briand C
European journal of cancer (Oxford, England : 1990) 2000 May;36(8):1043-9
European journal of cancer (Oxford, England : 1990) 2000 May;36(8):1043-9
The Smad4 activation domain (SAD) is a proline-rich, p300-dependent transcriptional activation domain.
de Caestecker MP, Yahata T, Wang D, Parks WT, Huang S, Hill CS, Shioda T, Roberts AB, Lechleider RJ
The Journal of biological chemistry 2000 Jan 21;275(3):2115-22
The Journal of biological chemistry 2000 Jan 21;275(3):2115-22
The Smad4 activation domain (SAD) is a proline-rich, p300-dependent transcriptional activation domain.
de Caestecker MP, Yahata T, Wang D, Parks WT, Huang S, Hill CS, Shioda T, Roberts AB, Lechleider RJ
The Journal of biological chemistry 2000 Jan 21;275(3):2115-22
The Journal of biological chemistry 2000 Jan 21;275(3):2115-22
Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53.
Zhang Y, Xiong Y
Molecular cell 1999 May;3(5):579-91
Molecular cell 1999 May;3(5):579-91
Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53.
Zhang Y, Xiong Y
Molecular cell 1999 May;3(5):579-91
Molecular cell 1999 May;3(5):579-91
Truncated N-terminal fragments of huntingtin with expanded glutamine repeats form nuclear and cytoplasmic aggregates in cell culture.
Cooper JK, Schilling G, Peters MF, Herring WJ, Sharp AH, Kaminsky Z, Masone J, Khan FA, Delanoy M, Borchelt DR, Dawson VL, Dawson TM, Ross CA
Human molecular genetics 1998 May;7(5):783-90
Human molecular genetics 1998 May;7(5):783-90
Truncated N-terminal fragments of huntingtin with expanded glutamine repeats form nuclear and cytoplasmic aggregates in cell culture.
Cooper JK, Schilling G, Peters MF, Herring WJ, Sharp AH, Kaminsky Z, Masone J, Khan FA, Delanoy M, Borchelt DR, Dawson VL, Dawson TM, Ross CA
Human molecular genetics 1998 May;7(5):783-90
Human molecular genetics 1998 May;7(5):783-90
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways.
Zhang Y, Xiong Y, Yarbrough WG
Cell 1998 Mar 20;92(6):725-34
Cell 1998 Mar 20;92(6):725-34
ARF promotes MDM2 degradation and stabilizes p53: ARF-INK4a locus deletion impairs both the Rb and p53 tumor suppression pathways.
Zhang Y, Xiong Y, Yarbrough WG
Cell 1998 Mar 20;92(6):725-34
Cell 1998 Mar 20;92(6):725-34
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of c-myc using c-myc Monoclonal Antibody (Product # MA5-12080) on Raji Cells.
- Submitted by
- Invitrogen Antibodies (provider)
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- Western blot was performed using c-Myc Monoclonal Antibody (9E10) (Product # MA5-12080) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates:Untransfected, 40 µg (Lane 1), empty vector control, 40 µg (Lane 2), His-H3-Myc (40 µg) (Lane 3), His-H3-Myc (20 µg) (Lane 4), Myc-p65-V5 (10 µg) (Lane 5), Myc-p65-V5 (5 µg) (Lane 6), p65-HA, 40 µg (Lane 7) and 25 ng of Positope (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). A ~65 kDa band corresponding to Myc-p65-V5 was observed in HEK293E transfected lysates on probing with the primary antibody (1:200 dilution) and detected by chemiluminescence with Goat Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Positope (Product # R90050) is a 53 kDa recombinant protein consisting of multiple epitope tags but lacks HA tag, which has been used as a positive control for c-Myc tag detection. No cross-reactivity was seen with HA-tagged p65 expressing lysate
Supportive validation
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- Invitrogen Antibodies (provider)
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- Immunofluorescent analysis of Myc tag was performed using 70% confluent His-H3-Myc transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Myc Tag Monoclonal Antibody (Product # MA5-12080) at 1:100 dilution and Histone H3 antibody (Product # 711055) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight, and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 (Product # A32728) and Goat anti-Rabbit IgG Alexa Fluor Plus 488 (Product # A32731) respectively at a dilution of 1:2000 for 45 minutes at room temperature. Panel a (Nuclei: Red) represents Myc tag. Panel b (Nuclei: Green) represents Histone H3. Panel c (Nuclei: Blue) represents ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel d represents the merged image showing the co-localization of nuclear signals in transfected cells. Panel e represents un-transfected HEK-293 cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
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- Invitrogen Antibodies (provider)
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- Formalin-fixed, paraffin-embedded human breast carcinoma stained with c-myc antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.
Supportive validation
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- Invitrogen Antibodies (provider)
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- Figure 2 Effect of gene silencing in gene and protein expression in gastric cancer cell lines A. YWHAE silencing induced MYC and CDC25B increased expression in GC cell lines. B. GC cells with (+) or without (-) YWHAE silencing, equal amounts of whole cell extracts were analyzed by western blot with the indicated antibodies. C. CDCD25B silencing did not alter MYC and YHWAE expression in GC cell lines. D. GC cells with (+) or without (-) CDC25B silencing; Equal amounts of whole cell extracts were analyzed by western blot with the indicated antibodies. E. MYC silencing induced the reduction of CDC25B expression and increasing of YHWAE expression in GC cell lines. F. GC cells with (+) or without (-) MYC silencing; equal amounts of whole cell extracts were analyzed by western blot with the indicated antibodies. siRNA control-transfected cells were used as a calibrator. Values of median and IQR are shown.
- Submitted by
- Invitrogen Antibodies (provider)
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- Figure 9 Protein and gene expression in gastric cancer. A. YWHAE protein expression B. YHWAE mRNA expression. C. CDC25B protein expression. D. CDC25B mRNA expression. E. MYC protein expression. F. MYC mRNA expression. G. Representative image of Western-blot. In all graphs, the expression in gastric tumors was normalized by matched non-neoplastic gastric tissue. T: tumor sample; N: normal mucosa sample; RQ: relative quantification. The whiskers indicate the minimum and maximum values. The dotted lines representes the 1.5 fold-change.
