Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- IC3696A - Provider product page
- Provider
- R&D Systems
- Product name
- Human c-Myc APC-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects endogenous human c-Myc and c-Myc tagged proteins in Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Antigen sequence
P01106
- Isotype
- IgG
- Antibody clone number
- 9.00E+10
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references A novel luciferase-based assay for the detection of Chimeric Antigen Receptors.
Gopalakrishnan R, Matta H, Choi S, Natarajan V, Prins R, Gong S, Zenunovic A, Narasappa N, Patel F, Prakash R, Chaudhary V, Sikri V, Chitnis SD, Kochegarov A, Wang D, Falat M, Kahn M, Keerthipati PS, Sharma N, Lenka J, Stieben TM, Braun J, Batra A, Purvis K, Ito K, Lee JH, Jeronimo A, Zamora HM, Membreno A, Qiu Q, Peshin S, Namburu L, Chaudhary PM
Scientific reports 2019 Feb 13;9(1):1957
Scientific reports 2019 Feb 13;9(1):1957
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of c-Myc in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human c-Myc APC-conjugated Monoclonal Antibody (Catalog # IC3696A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.