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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-58746 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SGTVYHNSQR ESRPVQQTTR RSVRRRGRTR VFLEQDRERE RRGTAYTPFS NSRLVSRITV EEGEESSRSS TAVRR Highest antigen sequence identity to the following orthologs: Mouse - 76%, Rat - 72%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RNF6 in control (vector only transfected HEK293T lysate) and RNF6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a RNF6 Polyclonal Antibody (Product # PA5-58746).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RNF6 in human cell line U-2 OS shows positivity in nuclear membrane. Samples were probed using a RNF6 Polyclonal Antibody (Product # PA5-58746).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RNF6 in human testis tissue shows moderate nuclear positivity in seminiferus ducts and leydig cells. Samples were probed using a RNF6 Polyclonal Antibody (Product # PA5-58746).
