20437-1-AP

antibody from Invitrogen Antibodies

Targeting: RNF6 DKFZp686P0776

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

20437-1-AP

Provider

Invitrogen Antibodies

Product name

RNF6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MNQSRSRSD GGSEETLPQD HNHHENERRW QQERLHREEA YYQFINELND EDYRLMRDHN LLGTPGEITS EELQQRLDGV KEQLASQPDL RDGTNYRDSE VPRESSHEDS LLEWLNTFRR TGNATRSGQN GNQTWRAVSR TNPNNGEFRF SLEIHVNHEN RGFEIHGEDY TDIPLSDSNR DHTANRQQRS TSPVARRTRS QTSVNFNGSS SNIPRTRLAS RGQNPAEGSF STLGRLRNGI GGAAGIPRAN ASRTNFSSHT NQSGGSELRQ REGQRFGAAH VWENGARSNV TVRNTNQRLE P (1-300 aa encoded by BC034688)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using RNF6 antibody 20437-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RNF6 (IP:20437-1-AP, 4ug; Detection:20437-1-AP 1:800) with mouse testis tissue lysate 4000ug.