GTX48763
antibody from GeneTex
Targeting: IL36RN
FIL1, FIL1(DELTA), FIL1D, IL-1F5, IL1F5, IL1HY1, IL1L1, IL1RP3, IL36RA, MGC29840
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- GTX48763 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48763, RRID:AB_11165175
- Product name
- IL1F5 antibody
- Antibody type
- Polyclonal
- Reactivity
- Bovine
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex's Protein-A Purified anti-bovine IL-1F5 antibody shows detection of recombinant bovine IL-1F5 at 17.1 kDa (arrowhead) raised in yeast. The identity of the band at 36 kDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1 µg/mL primary antibody overnight at 4°C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex's Protein-A Purified anti-bovine IL-1F5 antibody shows detection of recombinant bovine IL-1F5 at 17.1 kDa (arrowhead) raised in yeast. The identity of the band at 36 kDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA, and probed with 1 ?g/mL primary antibody overnight at 4¢XC. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.