Antibody data
- Antibody Data
- Antigen structure
- References [26]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX112794 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112794, RRID:AB_1950298
- Product name
- Fibronectin antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Improved Bioactivity of 3D Printed Porous Titanium Alloy Scaffold with Chitosan/Magnesium-Calcium Silicate Composite for Orthopaedic Applications.
Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
The natural compound 2,3,5,4'-tetrahydroxystilbene-2-O-β-d glucoside protects against adriamycin-induced nephropathy through activating the Nrf2-Keap1 antioxidant pathway.
TOP2A induces malignant character of pancreatic cancer through activating β-catenin signaling pathway.
CHD4-mediated loss of E-cadherin determines metastatic ability in triple-negative breast cancer cells.
Runx3 inhibits melanoma cell migration through regulation of cell shape change.
Autologous Adipose-Derived Tissue Matrix Part I: Biologic Characteristics.
Neutralization of IL-8 decreases tumor PMN-MDSCs and reduces mesenchymalization of claudin-low triple-negative breast cancer.
Downregulation of miR-199a/b-5p is associated with GCNT2 induction upon epithelial-mesenchymal transition in colon cancer.
Glucocorticoid receptor signalling activates YAP in breast cancer.
Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
Simulated physiological stretch increases expression of extracellular matrix proteins in human bladder smooth muscle cells via integrin α4/αv-FAK-ERK1/2 signaling pathway.
Short-term EGFR blockade enhances immune-mediated cytotoxicity of EGFR mutant lung cancer cells: rationale for combination therapies.
Oxystressed tumor microenvironment potentiates epithelial to mesenchymal transition and alters cellular bioenergetics towards cancer progression.
Overexpression of lipocalin 2 in human cervical cancer enhances tumor invasion.
Aberrant DNA hypomethylation of miR-196b contributes to migration and invasion of oral cancer.
LRIG1 opposes epithelial-to-mesenchymal transition and inhibits invasion of basal-like breast cancer cells.
Targeting Estrogen Receptor Signaling with Fulvestrant Enhances Immune and Chemotherapy-Mediated Cytotoxicity of Human Lung Cancer.
Contrasting effects of stanniocalcin-related polypeptides on macrophage foam cell formation and vascular smooth muscle cell migration.
Dexamethasone-induced cellular tension requires a SGK1-stimulated Sec5-GEF-H1 interaction.
Atypical ubiquitin E3 ligase complex Skp1-Pam-Fbxo45 controls the core epithelial-to-mesenchymal transition-inducing transcription factors.
Comprehensive proteome quantification reveals NgBR as a new regulator for epithelial-mesenchymal transition of breast tumor cells.
MEF2B mutations in non-Hodgkin lymphoma dysregulate cell migration by decreasing MEF2B target gene activation.
Titania nanotubes dimensions-dependent protein adsorption and its effect on the growth of osteoblasts.
Immunohistochemical analysis of thyroid follicular neoplasms and BRAF mutation correlation.
Analysis of molecular mechanisms and anti-tumoural effects of zoledronic acid in breast cancer cells.
Tsai CH, Hung CH, Kuo CN, Chen CY, Peng YN, Shie MY
Materials (Basel, Switzerland) 2019 Jan 9;12(2)
Materials (Basel, Switzerland) 2019 Jan 9;12(2)
Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
Pei YF, Xu XN, Wang ZF, Wang FW, Wu WD, Geng JF, Liu XQ
The American journal of pathology 2019 Feb 5;
The American journal of pathology 2019 Feb 5;
The natural compound 2,3,5,4'-tetrahydroxystilbene-2-O-β-d glucoside protects against adriamycin-induced nephropathy through activating the Nrf2-Keap1 antioxidant pathway.
Lin EY, Bayarsengee U, Wang CC, Chiang YH, Cheng CW
Environmental toxicology 2018 Jan;33(1):72-82
Environmental toxicology 2018 Jan;33(1):72-82
TOP2A induces malignant character of pancreatic cancer through activating β-catenin signaling pathway.
Pei YF, Yin XM, Liu XQ
Biochimica et biophysica acta. Molecular basis of disease 2018 Jan;1864(1):197-207
Biochimica et biophysica acta. Molecular basis of disease 2018 Jan;1864(1):197-207
CHD4-mediated loss of E-cadherin determines metastatic ability in triple-negative breast cancer cells.
Luo CW, Wu CC, Chang SJ, Chang TM, Chen TY, Chai CY, Chang CL, Hou MF, Pan MR
Experimental cell research 2018 Feb 1;363(1):65-72
Experimental cell research 2018 Feb 1;363(1):65-72
Runx3 inhibits melanoma cell migration through regulation of cell shape change.
Zhang X, Wang L, Zeng X, Fujita T, Liu W
Cell biology international 2017 Sep;41(9):1048-1055
Cell biology international 2017 Sep;41(9):1048-1055
Autologous Adipose-Derived Tissue Matrix Part I: Biologic Characteristics.
Schendel SA
Aesthetic surgery journal 2017 Oct 1;37(9):1062-1068
Aesthetic surgery journal 2017 Oct 1;37(9):1062-1068
Neutralization of IL-8 decreases tumor PMN-MDSCs and reduces mesenchymalization of claudin-low triple-negative breast cancer.
Dominguez C, McCampbell KK, David JM, Palena C
JCI insight 2017 Nov 2;2(21)
JCI insight 2017 Nov 2;2(21)
Downregulation of miR-199a/b-5p is associated with GCNT2 induction upon epithelial-mesenchymal transition in colon cancer.
Chao CC, Wu PH, Huang HC, Chung HY, Chou YC, Cai BH, Kannagi R
FEBS letters 2017 Jul;591(13):1902-1917
FEBS letters 2017 Jul;591(13):1902-1917
Glucocorticoid receptor signalling activates YAP in breast cancer.
Sorrentino G, Ruggeri N, Zannini A, Ingallina E, Bertolio R, Marotta C, Neri C, Cappuzzello E, Forcato M, Rosato A, Mano M, Bicciato S, Del Sal G
Nature communications 2017 Jan 19;8:14073
Nature communications 2017 Jan 19;8:14073
Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
Wang LT, Chiou SS, Chai CY, Hsi E, Chiang CM, Huang SK, Wang SN, Yokoyama KK, Hsu SH
Oncogene 2017 Aug;36(31):4405-4414
Oncogene 2017 Aug;36(31):4405-4414
Simulated physiological stretch increases expression of extracellular matrix proteins in human bladder smooth muscle cells via integrin α4/αv-FAK-ERK1/2 signaling pathway.
Chen S, Peng C, Wei X, Luo D, Lin Y, Yang T, Jin X, Gong L, Li H, Wang K
World journal of urology 2017 Aug;35(8):1247-1254
World journal of urology 2017 Aug;35(8):1247-1254
Short-term EGFR blockade enhances immune-mediated cytotoxicity of EGFR mutant lung cancer cells: rationale for combination therapies.
Dominguez C, Tsang KY, Palena C
Cell death & disease 2016 Sep 29;7(9):e2380
Cell death & disease 2016 Sep 29;7(9):e2380
Oxystressed tumor microenvironment potentiates epithelial to mesenchymal transition and alters cellular bioenergetics towards cancer progression.
Sridaran D, Ramamoorthi G, MahaboobKhan R, Kumpati P
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Oct;37(10):13307-13322
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Oct;37(10):13307-13322
Overexpression of lipocalin 2 in human cervical cancer enhances tumor invasion.
Chung IH, Wu TI, Liao CJ, Hu JY, Lin YH, Tai PJ, Lai CH, Lin KH
Oncotarget 2016 Mar 8;7(10):11113-26
Oncotarget 2016 Mar 8;7(10):11113-26
Aberrant DNA hypomethylation of miR-196b contributes to migration and invasion of oral cancer.
Hou YY, You JJ, Yang CM, Pan HW, Chen HC, Lee JH, Lin YS, Liou HH, Liu PF, Chi CC, Ger LP, Tsai KW
Oncology letters 2016 Jun;11(6):4013-4021
Oncology letters 2016 Jun;11(6):4013-4021
LRIG1 opposes epithelial-to-mesenchymal transition and inhibits invasion of basal-like breast cancer cells.
Yokdang N, Hatakeyama J, Wald JH, Simion C, Tellez JD, Chang DZ, Swamynathan MM, Chen M, Murphy WJ, Carraway Iii KL, Sweeney C
Oncogene 2016 Jun 2;35(22):2932-47
Oncogene 2016 Jun 2;35(22):2932-47
Targeting Estrogen Receptor Signaling with Fulvestrant Enhances Immune and Chemotherapy-Mediated Cytotoxicity of Human Lung Cancer.
Hamilton DH, Griner LM, Keller JM, Hu X, Southall N, Marugan J, David JM, Ferrer M, Palena C
Clinical cancer research : an official journal of the American Association for Cancer Research 2016 Dec 15;22(24):6204-6216
Clinical cancer research : an official journal of the American Association for Cancer Research 2016 Dec 15;22(24):6204-6216
Contrasting effects of stanniocalcin-related polypeptides on macrophage foam cell formation and vascular smooth muscle cell migration.
Yamamoto K, Tajima Y, Hasegawa A, Takahashi Y, Kojima M, Watanabe R, Sato K, Shichiri M, Watanabe T
Peptides 2016 Aug;82:120-127
Peptides 2016 Aug;82:120-127
Dexamethasone-induced cellular tension requires a SGK1-stimulated Sec5-GEF-H1 interaction.
Wang HL, Yang CH, Lee HH, Kuo JC, Hur SS, Chien S, Lee OK, Hung SC, Chang ZF
Journal of cell science 2015 Oct 15;128(20):3757-68
Journal of cell science 2015 Oct 15;128(20):3757-68
Atypical ubiquitin E3 ligase complex Skp1-Pam-Fbxo45 controls the core epithelial-to-mesenchymal transition-inducing transcription factors.
Xu M, Zhu C, Zhao X, Chen C, Zhang H, Yuan H, Deng R, Dou J, Wang Y, Huang J, Chen Q, Jiang B, Yu J
Oncotarget 2015 Jan 20;6(2):979-94
Oncotarget 2015 Jan 20;6(2):979-94
Comprehensive proteome quantification reveals NgBR as a new regulator for epithelial-mesenchymal transition of breast tumor cells.
Zhao B, Xu B, Hu W, Song C, Wang F, Liu Z, Ye M, Zou H, Miao QR
Journal of proteomics 2015 Jan 1;112:38-52
Journal of proteomics 2015 Jan 1;112:38-52
MEF2B mutations in non-Hodgkin lymphoma dysregulate cell migration by decreasing MEF2B target gene activation.
Pon JR, Wong J, Saberi S, Alder O, Moksa M, Grace Cheng SW, Morin GB, Hoodless PA, Hirst M, Marra MA
Nature communications 2015 Aug 6;6:7953
Nature communications 2015 Aug 6;6:7953
Titania nanotubes dimensions-dependent protein adsorption and its effect on the growth of osteoblasts.
Yang W, Xi X, Shen X, Liu P, Hu Y, Cai K
Journal of biomedical materials research. Part A 2014 Oct;102(10):3598-608
Journal of biomedical materials research. Part A 2014 Oct;102(10):3598-608
Immunohistochemical analysis of thyroid follicular neoplasms and BRAF mutation correlation.
Atik E, Guray M, Gunesacar R, Ozgur T, Canda T
Indian journal of cancer 2014 Jan-Mar;51(1):63-8
Indian journal of cancer 2014 Jan-Mar;51(1):63-8
Analysis of molecular mechanisms and anti-tumoural effects of zoledronic acid in breast cancer cells.
Insalaco L, Di Gaudio F, Terrasi M, Amodeo V, Caruso S, Corsini LR, Fanale D, Margarese N, Santini D, Bazan V, Russo A
Journal of cellular and molecular medicine 2012 Sep;16(9):2186-95
Journal of cellular and molecular medicine 2012 Sep;16(9):2186-95
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hela 5% SDS PAGE GTX112794 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fibronectin antibody [N1N2], N-term detects Fibronectin protein by western blot analysis. Whole cell extracts (30 £gg) was separated by 5 % SDS-PAGE, and blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted by 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fibronectin antibody [N1N2], N-term detects Fibronectin protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 5 % SDS-PAGE, and blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted by 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fibronectin antibody [N1N2] detects Fibronectin protein by western blot analysis. Mouse tissue extracts (50 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HepG2 whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fibronectin antibody detects Fibronectin protein by Western blot analysis. Whole cell extracts (30 £gg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody (GTX112794) at a dilution of 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibronectin (GTX112794) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fibronectin antibody [N1N2], N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 £gg whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 £gg of pre-immune rabbit IgG C : Immunoprecipitation of Fibronectin by 3 £gg of Fibronectin antibody [N1N2], N-term (GTX112794) 5% SDS-PAGE The immunoprecipitated Fibronectin protein was detected by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fibronectin antibody [N1N2], N-term detects FN1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Fibronectin antibody [N1N2], N-term (GTX112794) dilution: 1:500.