LS-C355423

antibody from LSBio

Targeting: FN1 CIG, FINC, GFND2, LETS, MSF

Immunocytochemistry

Immunoprecipitation

Radioimmunoassay

Antibody data

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Validation data

Product number: LS-C355423 - Provider product page
Provider: LSBio
Product name: FN1 / Fibronectin Antibody (clone 3F12) LS-C355423
Antibody type: Monoclonal
Description: Protein A purified
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 3F12
Storage: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

FN1 protein structure - LS-C355423 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Direct ELISA analysis of Ferritin was performed by coating wells of a 96-well plate with 100ul per well of Human Ferritin or BSA diluted in carbonate/bicarbonate buffer, starting at a concentration of 1 µg/mL and serially diluting 2-fold to a concentration of 2ng/ml, overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 100ul per well of a mouse anti-ferritin monoclonal antibody at a concentration of 1 µg/mL for 1 hour at room temperature. The plate was washed, then incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:25000 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB substrate for 10 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid, and absorbances were read on a spectrophotometer at 450-550nm.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Sandwich ELISA of Fibronectin was performed by coating wells of a 96-well plate with 100ul of an fibronectin chicken polyclonal antibody diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 100ul of StartingBlock T20 (TBS) Blocking Buffer for 2 hours, and 100ul of recombinant human plasma fibronectin protein ranging from 1.6-1000ng/ml were incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of a fibronectin mouse monoclonal antibody diluted to a concentration of 1 µg/mL was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of a Streptavidin-HRP was incubated for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Direct ELISA analysis of Ferritin was performed by coating wells of a 96-well plate with 100ul per well of Human Ferritin or BSA diluted in carbonate/bicarbonate buffer, starting at a concentration of 1 µg/mL and serially diluting 2-fold to a concentration of 2ng/ml, overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 100ul per well of a mouse anti-ferritin monoclonal antibody at a concentration of 1 µg/mL for 1 hour at room temperature. The plate was washed, then incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:25000 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB substrate for 10 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid, and absorbances were read on a spectrophotometer at 450-550nm.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Sandwich ELISA of Fibronectin was performed by coating wells of a 96-well plate with 100ul of an fibronectin chicken polyclonal antibody diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 100ul of StartingBlock T20 (TBS) Blocking Buffer for 2 hours, and 100ul of recombinant human plasma fibronectin protein ranging from 1.6-1000ng/ml were incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of a fibronectin mouse monoclonal antibody diluted to a concentration of 1 µg/mL was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of a Streptavidin-HRP was incubated for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Sandwich ELISA of Fibronectin was performed by coating wells of a 96-well plate with 100ul of an fibronectin chicken polyclonal antibody diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer overnight at 4C. Wells were blocked with 100ul of StartingBlock T20 (TBS) Blocking Buffer for 2 hours, and 100ul of recombinant human plasma fibronectin protein ranging from 1.6-1000ng/ml were incubated for 1 hour at room temperature. The plate was washed with 1X TBST, and 100ul per well of a fibronectin mouse monoclonal antibody diluted to a concentration of 1 µg/mL was added to each well for 1 hour at room temperature. The plate was washed, and 100ul per well of a Streptavidin-HRP was incubated for 30 minutes. Detection was performed using 1-Step Ultra TMB Substrate, followed by Stop Solution. Absorbances were read on a spectrophotometer at 450-550nm.