H00002335-B01P

antibody from Abnova Corporation

Targeting: FN1 CIG, FINC, GFND2, LETS, MSF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002335-B01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002335-B01P, RRID:AB_1573618

Product name

FN1 purified MaxPab mouse polyclonal antibody (B01P)

Antibody type

Polyclonal

Description

Mouse polyclonal antibody raised against a full-length human FN1 protein.

Antigen sequence

MSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNY
KIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEA
TCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGW
RCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNT
NVNCPIECFMPLDVQADREDSRE


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

FN1 MaxPab polyclonal antibody. Western Blot analysis of FN1 expression in human kidney.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of FN1 expression in transfected 293T cell line (H00002335-T01) by FN1 MaxPab polyclonal antibody.Lane 1: FN1 transfected lysate(23.21 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to FN1 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between HGF and FN1. HeLa cells were stained with anti-HGF rabbit purified polyclonal 1:1200 and anti-FN1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)