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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- V7408 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Fibronectin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 616
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)
- Storage
- Store the Fibronectin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Submitted references Fibronectin fragments and the cleaving enzyme ADAM-8 in the degenerative human intervertebral disc.
Matrix metalloproteinase expression in human retinal microvascular cells.
Ruel N, Markova DZ, Adams SL, Scanzello C, Cs-Szabo G, Gerard D, Shi P, Anderson DG, Zack M, An HS, Chen D, Zhang Y
Spine 2014 Jul 15;39(16):1274-9
Spine 2014 Jul 15;39(16):1274-9
Matrix metalloproteinase expression in human retinal microvascular cells.
Grant MB, Caballero S, Tarnuzzer RW, Bass KE, Ljubimov AV, Spoerri PE, Galardy RE
Diabetes 1998 Aug;47(8):1311-7
Diabetes 1998 Aug;47(8):1311-7
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human prostate carcinoma with Fibronectin antibody (clone 616). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling prior to testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free Fibronectin antibody (clone 616) as confirmation of integrity and purity.
