NBP1-31369

antibody from Novus Biologicals

Targeting: FN1 CIG, FINC, GFND2, LETS, MSF

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-31369

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-31369, RRID:AB_10003791

Product name

Rabbit Polyclonal Fibronectin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Fibronectin/Anastellin Antibody [NBP1-31369] - Mouse tissue extracts (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Fibronectin/Anastellin Antibody [NBP1-31369] - Whole cell extracts (30 ug) was separated by 5 % SDS-PAGE, and blotted with Fibronectin antibody [N1N2], N-term diluted by 1:1000

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Fibronectin Antibody [NBP1-31369] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: Fibronectin Antibody [NBP1-31369] - Indirect ELISA analysis was performed by coating plate with 100 uL of recombinant Fibronectin protein at concentration of 10 ug/mL. The coated protein is detected with Fibronectin antibody [N1N2], N-term at rangeing from 0.5 to 140 ng/mL.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: Fibronectin Antibody [NBP1-31369] - Competitive ELISA detection of human fibronectin in plasma. Recombinant or purified native Fibronectin protein was coated to an ELISA plate at concentration of 10 ug/ml. Human plasma was applied as the competitors and was serial diluted at 1, 20, 200, 2000, 20000, 200000X dilutions. The primary antibody concentration was diluted to 14 ng/mL. Rabbit IgG antibody (HRP) was diluted at 1:2,000 and used to detect the primary antibody

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: Fibronectin/Anastellin Antibody [NBP1-31369] - N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of Fibronectin by 3 ug of Fibronectin antibody [N1N2].

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Fibronectin/Anastellin Antibody [NBP1-31369] - Paraffin-embedded hepatoma. Fibronectin antibody [N1N2], N-term dilution: 1:500.