Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-31369 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31369, RRID:AB_10003791
- Product name
- Rabbit Polyclonal Fibronectin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fibronectin/Anastellin Antibody [NBP1-31369] - Mouse tissue extracts (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fibronectin/Anastellin Antibody [NBP1-31369] - Whole cell extracts (30 ug) was separated by 5 % SDS-PAGE, and blotted with Fibronectin antibody [N1N2], N-term diluted by 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fibronectin Antibody [NBP1-31369] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Fibronectin Antibody [NBP1-31369] - Indirect ELISA analysis was performed by coating plate with 100 uL of recombinant Fibronectin protein at concentration of 10 ug/mL. The coated protein is detected with Fibronectin antibody [N1N2], N-term at rangeing from 0.5 to 140 ng/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Fibronectin Antibody [NBP1-31369] - Competitive ELISA detection of human fibronectin in plasma. Recombinant or purified native Fibronectin protein was coated to an ELISA plate at concentration of 10 ug/ml. Human plasma was applied as the competitors and was serial diluted at 1, 20, 200, 2000, 20000, 200000X dilutions. The primary antibody concentration was diluted to 14 ng/mL. Rabbit IgG antibody (HRP) was diluted at 1:2,000 and used to detect the primary antibody
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Fibronectin/Anastellin Antibody [NBP1-31369] - N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of Fibronectin by 3 ug of Fibronectin antibody [N1N2].
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Fibronectin/Anastellin Antibody [NBP1-31369] - Paraffin-embedded hepatoma. Fibronectin antibody [N1N2], N-term dilution: 1:500.