RQ5531

antibody from NSJ Bioreagents

Targeting: FN1 CIG, FINC, GFND2, LETS, MSF

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5531

Provider

NSJ Bioreagents

Product name

FN1 Antibody / Fibronectin

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human

Host

Rabbit

Vial size

100 ug

Storage

After reconstitution, the Fibronectin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human U-87 MG cell lysate with Fibronectin antibody. Predicted molecular weight ~220 kDa (monomer) but may be observed at a higher molecular weight due to glycosylation.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) plasma, 2) HCCT and 3) HCCP cell lysate with Fibronectin antibody. Predicted molecular weight ~220 kDa (monomer) but may be observed at a higher molecular weight due to glycosylation.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human adrenocortical adenoma tissue with Fibronectin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colorectal adenocarcinoma tissue with Fibronectin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HeLa cells with Fibronectin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fibronectin antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human Caco-2 cells with Fibronectin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fibronectin antibody.