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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- RQ5531 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Fibronectin Antibody / FN1
- Antibody type
- Polyclonal
- Description
- This highly specific Fibronectin antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry/Direct ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Fibronectin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human U-87 MG cell lysate with Fibronectin antibody. Predicted molecular weight ~220 kDa (monomer) but may be observed at a higher molecular weight due to glycosylation.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) plasma, 2) HCCT and 3) HCCP cell lysate with Fibronectin antibody. Predicted molecular weight ~220 kDa (monomer) but may be observed at a higher molecular weight due to glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human adrenocortical adenoma tissue with Fibronectin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human colorectal adenocarcinoma tissue with Fibronectin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HeLa cells with Fibronectin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fibronectin antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human Caco-2 cells with Fibronectin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fibronectin antibody.
