Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1918 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Fibronectin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Fibronectin in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A NOTCH feed-forward loop drives reprogramming from adrenergic to mesenchymal state in neuroblastoma.
Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury.
Neuroblastoma is composed of two super-enhancer-associated differentiation states.
PDGFRβ expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation.
Semen clusterin is a novel DC-SIGN ligand.
Assembly of fibrillin microfibrils governs extracellular deposition of latent TGF beta.
van Groningen T, Akogul N, Westerhout EM, Chan A, Hasselt NE, Zwijnenburg DA, Broekmans M, Stroeken P, Haneveld F, Hooijer GKJ, Savci-Heijink CD, Lakeman A, Volckmann R, van Sluis P, Valentijn LJ, Koster J, Versteeg R, van Nes J
Nature communications 2019 Apr 4;10(1):1530
Nature communications 2019 Apr 4;10(1):1530
Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury.
Vishwakarma SK, Bardia A, Lakkireddy C, Paspala SAB, Khan AA
Frontiers in bioengineering and biotechnology 2018;6:150
Frontiers in bioengineering and biotechnology 2018;6:150
Neuroblastoma is composed of two super-enhancer-associated differentiation states.
van Groningen T, Koster J, Valentijn LJ, Zwijnenburg DA, Akogul N, Hasselt NE, Broekmans M, Haneveld F, Nowakowska NE, Bras J, van Noesel CJM, Jongejan A, van Kampen AH, Koster L, Baas F, van Dijk-Kerkhoven L, Huizer-Smit M, Lecca MC, Chan A, Lakeman A, Molenaar P, Volckmann R, Westerhout EM, Hamdi M, van Sluis PG, Ebus ME, Molenaar JJ, Tytgat GA, Westerman BA, van Nes J, Versteeg R
Nature genetics 2017 Aug;49(8):1261-1266
Nature genetics 2017 Aug;49(8):1261-1266
PDGFRβ expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation.
Hewitt KJ, Shamis Y, Knight E, Smith A, Maione A, Alt-Holland A, Sheridan SD, Haggarty SJ, Garlick JA
Journal of cell science 2012 May 1;125(Pt 9):2276-87
Journal of cell science 2012 May 1;125(Pt 9):2276-87
Semen clusterin is a novel DC-SIGN ligand.
Sabatte J, Faigle W, Ceballos A, Morelle W, Rodríguez Rodrígues C, Remes Lenicov F, Thépaut M, Fieschi F, Malchiodi E, Fernández M, Arenzana-Seisdedos F, Lortat-Jacob H, Michalski JC, Geffner J, Amigorena S
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):5299-309
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):5299-309
Assembly of fibrillin microfibrils governs extracellular deposition of latent TGF beta.
Massam-Wu T, Chiu M, Choudhury R, Chaudhry SS, Baldwin AK, McGovern A, Baldock C, Shuttleworth CA, Kielty CM
Journal of cell science 2010 Sep 1;123(Pt 17):3006-18
Journal of cell science 2010 Sep 1;123(Pt 17):3006-18
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Fibronectin in A549 Human Cell Line. Fibronectin was detected in immersion fixed A549 human lung carcinoma cell line untreated (top panel) or treated with 10 ng/mL TGF-beta (Catalog # 240-B, lower panel) for 48 hours using Sheep Anti-Human Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Fibronectin in Human Liver. Fibronectin was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.