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12609-1-AP

antibody from Invitrogen Antibodies

Targeting: WT1 AWT1, GUD, NPHS4, WAGR, WIT-2

Provider product page for 12609-1-AP

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [0]
Comments [0]
Validations
Western blot [9]
Immunohistochemistry [1]
Flow cytometry [1]
Other assay [1]

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Validation data

Product number: 12609-1-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: WT1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 150 µL
Concentration: 0.21 mg/mL
Storage: -20°C

WT1 protein structure - 12609-1-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: A431 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: MCF7 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Human kidney tissue were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Human liver tissue were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Rat kidney tissue were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.