Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF7925 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CTRP3/C1qTNF3/CORS26 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CTRP3/C1qTNF3/CORS26 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse CTRP3/C1qTNF3/CORS26 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q9BXJ4- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CTRP3/C1qTNF3/CORS26 by Western Blot. Western blot shows lysates of human mesenchymal stem cells either undifferentiated or differentiated into adipocytes. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CTRP3/C1qTNF3/CORS26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7925) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CTRP3/C1qTNF3/CORS26 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CTRP3/C1qTNF3/CORS26 in Human Mesenchymal Stem Cells. CTRP3/C1qTNF3/CORS26 was detected in immersion fixed human mesenchymal stem cells differentiated (upper panel) or not (lower panel) into adipocytes using Sheep Anti-Human CTRP3/C1qTNF3/CORS26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7925) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue. Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
