PA5-17891

antibody from Invitrogen Antibodies

Targeting: TP53 LFS1, p53

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17891

Provider

Invitrogen Antibodies

Product name

Phospho-p53 (Ser392) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Phospho-p53 (Ser392) Polyclonal Antibody (Product # PA5-17891) and a 53kDa band corresponding to phospho-Cellular tumor antigen p53 was observed across all cell lines tested and was upregulated upon various treatments. Uncharacterized bands (*) were also observed around ~18kDa and ~40 kDa in HCT116 and A549 cell lines, respectively. Whole-cell extracts (30 µg lysate) of HCT 116 (Lane 1), HCT 116 treated with Etoposide (50 micromolar for 24h) (Lane 2), MCF7 (Lane 3), MCF7 treated with Etoposide (50 micromolar for 6h) (Lane 4), A549 (Lane 5), A549 treated with Doxorubicin (250 nanomolar for 24h) (Lane 6), NIH/3T3 (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).