Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-17891 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-p53 (Ser392) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody. This antibody is not cross-reactive with p53 phosphorylated at other sites.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-Phospho-p53 (Ser392) Polyclonal Antibody (Product # PA5-17891) and a 53kDa band corresponding to phospho-Cellular tumor antigen p53 was observed across all cell lines tested and was upregulated upon various treatments. Uncharacterized bands (*) were also observed around ~18kDa and ~40 kDa in HCT116 and A549 cell lines, respectively. Whole-cell extracts (30 µg lysate) of HCT 116 (Lane 1), HCT 116 treated with Etoposide (50 micromolar for 24h) (Lane 2), MCF7 (Lane 3), MCF7 treated with Etoposide (50 micromolar for 6h) (Lane 4), A549 (Lane 5), A549 treated with Doxorubicin (250 nanomolar for 24h) (Lane 6), NIH/3T3 (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).