PA5-17894

antibody from Invitrogen Antibodies

Targeting: TP53 LFS1, p53

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17894

Provider

Invitrogen Antibodies

Product name

Phospho-p53 (Ser20) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with p53 phosphorylated at other sites.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

33 µg/mL

Storage

-20°C

References

Submitted references

Herpes Simplex Virus 1 Manipulates Host Cell Antiviral and Proviral DNA Damage Responses.

Mertens ME, Knipe DM
mBio 2021 Feb 9;12(1)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Phospho-p53 (Ser20) was performed using 70% confluent log phase HCT116 cells treated with etoposide. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-p53(Ser20) Polyclonal Antibody (Product # PA5-17894) at 1:100 dilution in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034 ) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents the untreated cells with relatively lower expression of Phospho-p53(Ser20). Panel f shows control cells with no primary antibody to assess background. The images were captured at 60X magnification.