PB9008

antibody from Boster Biological Technology

Targeting: TP53 LFS1, p53

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

PB9008

Provider

Boster Biological Technology

Product name

Anti-P53/TP53 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for p53/TP53 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, ICC/IF, FCM. Reactive species: Human. p53/TP53 information: Molecular Weight: 43653 MW; Subcellular Localization: Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2. Translocates to mitochondria upon oxidative stress; Tissue Specificity: Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

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Punicalagin Attenuates Neuronal Apoptosis by Upregulating 5-Hydroxymethylcytosine in the Diabetic Mouse Brain.

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Ferritinophagy-Mediated ROS Production Contributed to Proliferation Inhibition, Apoptosis, and Ferroptosis Induction in Action of Mechanism of 2-Pyridylhydrazone Dithiocarbamate Acetate.

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Biomedicines 2020 Nov 4;8(11)

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Zhang H, Liu M, Zhang H, Cao S, Li Y, Jiang S, Song Y, Liu S
BMC veterinary research 2020 Aug 24;16(1):303

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p53-Mediated PI3K/AKT/mTOR Pathway Played a Role in Ptox(Dpt)-Induced EMT Inhibition in Liver Cancer Cell Lines.

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Cisatracurium-induced proliferation impairment and death of colorectal cancer cells, HCT116 is mediated by p53 dependent intrinsic apoptotic pathway in vitro.

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Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2017 Jul;91:320-329

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Antiproliferative and Apoptosis-inducing Effect of exo-Protoporphyrin IX based Sonodynamic Therapy on Human Oral Squamous Cell Carcinoma.

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Fra-1 is upregulated in lung cancer tissues and inhibits the apoptosis of lung cancer cells by the P53 signaling pathway.

Zhong G, Chen X, Fang X, Wang D, Xie M, Chen Q
Oncology reports 2016 Jan;35(1):447-53

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Lactotransferrin expression is downregulated and affects the mitogen-activated protein kinase pathway in gastric cancer.

Luo G, Zhou Y, Yi W, Yi H
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Cisplatin-induced senescence in ovarian cancer cells is mediated by GRP78.

Li W, Wang W, Dong H, Li Y, Li L, Han L, Han Z, Wang S, Ma D, Wang H
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CD38 is highly expressed and affects the PI3K/Akt signaling pathway in cervical cancer.

Liao S, Xiao S, Zhu G, Zheng D, He J, Pei Z, Li G, Zhou Y
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Nutlin-3 downregulates p53 phosphorylation on serine392 and induces apoptosis in hepatocellular carcinoma cells.

Shi X, Liu J, Ren L, Mao N, Tan F, Ding N, Yang J, Li M
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Targeting of histone deacetylases to reactivate tumour suppressor genes and its therapeutic potential in a human cervical cancer xenograft model.

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Sulfite exposure-induced hepatocyte death is not associated with alterations in p53 protein expression.

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Ran GTPase protein promotes human pancreatic cancer proliferation by deregulating the expression of Survivin and cell cycle proteins.

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Rao Y, Wang H, Fan L, Chen G
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Apoptosis induced by baicalin involving up-regulation of P53 and bax in MCF-7 cells.

Wang N, Tang LJ, Zhu GQ, Peng DY, Wang L, Sun FN, Li QL
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Knockdown of survivin and upregulation of p53 gene expression by small interfering RNA induces apoptosis in human gastric carcinoma cell line SGC-823.

Lu YH, Wang K, He R, Xi T
Cancer biotherapy & radiopharmaceuticals 2008 Dec;23(6):727-34

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Positional and expressive alteration of prohibitin during the induced differentiation of human hepatocarcinoma SMMC-7721 cells.

Xu DH, Tang J, Li QF, Shi SL, Chen XF, Liang Y
World journal of gastroenterology 2008 Aug 28;14(32):5008-14

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Fluorescent nanoparticle-based indirect immunofluorescence microscopy for detection of Mycobacterium tuberculosis.

Qin D, He X, Wang K, Zhao XJ, Tan W, Chen J
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ZNF307, a novel zinc finger gene suppresses p53 and p21 pathway.

Li J, Wang Y, Fan X, Mo X, Wang Z, Li Y, Yin Z, Deng Y, Luo N, Zhu C, Liu M, Ma Q, Ocorr K, Yuan W, Wu X
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Safrole oxide induces apoptosis in A549 human lung cancer cells.

Du A, Zhang S, Miao J, Zhao B
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Integrin beta4 mAb inhibited apoptosis induced by deprivation of growth factors in vascular endothelial cells.

Zhao KW, Zhao QT, Zhang SL, Miao JY
Acta pharmacologica Sinica 2004 Jun;25(6):733-7

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of P53 using anti-P53 antibody (PB9008). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P53 antigen affinity purified polyclonal antibody (Catalog # PB9008) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P53 at approximately 53KD. The expected band size for P53 is at 53KD.

Additional image